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首页> 外文期刊>Cornea >Regulation of membrane-associated mucins in the human corneal epithelial cells by dexamethasone.
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Regulation of membrane-associated mucins in the human corneal epithelial cells by dexamethasone.

机译：地塞米松对人角膜上皮细胞中膜相关粘蛋白的调节。

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摘要

PURPOSE: To study the influence of dexamethasone on membrane-associated mucins produced by human corneal epithelial cells. METHODS: Human corneal epithelial cells were cultured in medium supplemented with various concentrations of dexamethasone (ranging from 10 to 10 M). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis using monoclonal antibodies specific for human MUC1 (HMFG-1), MUC4 (1G8), and MUC16 (OC125) were performed to evaluate the effect of dexamethasone on membrane-associated mucin expression. The effect of glucocorticoid receptor antagonist (RU38486) on dexamethasone-induced mucin expression was estimated. RESULTS: RT-PCR revealed that MUC1 and MUC16 gene expression were upregulated 48 hours after addition of dexamethasone and that MUC4 gene expression was downregulated in the same condition. Western blot analysis showed that MUC1 and MUC16 proteins were increased after addition of dexamethasone. However, MUC4 was not detected by anti-MUC4 monoclonal antibody (1G8) for ASGP-2 under our conditions. Treatment with RU38486 inhibited the changes of MUC1, MUC4, and MUC16 by dexamethasone; thus, the effect of dexamethasone on mucin expression is mediated by glucocorticoid receptors. CONCLUSIONS: This study shows that MUC1, MUC4, and MUC16 are regulated differently by dexamethasone in human corneal epithelial cells. External application of dexamethasone might affect the precorneal mucin.
机译：目的：研究地塞米松对人角膜上皮细胞产生的膜相关黏蛋白的影响。方法：在补充了各种浓度地塞米松（10至10 M）的培养基中培养人角膜上皮细胞。进行逆转录聚合酶链反应（RT-PCR）和Western blot分析，使用对人类MUC1（HMFG-1），MUC4（1G8）和MUC16（OC125）特异的单克隆抗体评估地塞米松对与膜相关的作用粘蛋白表达。估计了糖皮质激素受体拮抗剂（RU38486）对地塞米松诱导的粘蛋白表达的影响。结果：RT-PCR显示，地塞米松添加48小时后MUC1和MUC16基因表达上调，而在相同条件下MUC4基因表达下调。 Western印迹分析表明，加入地塞米松后，MUC1和MUC16蛋白增加。但是，在我们的条件下，ASGP-2的抗MUC4单克隆抗体（1G8）未检测到MUC4。 RU38486处理可抑制地塞米松对MUC1，MUC4和MUC16的影响。因此，地塞米松对粘蛋白表达的作用是由糖皮质激素受体介导的。结论：这项研究表明，地塞米松在人角膜上皮细胞中对MUC1，MUC4和MUC16的调控不同。地塞米松的外用可能会影响角膜前粘蛋白。

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