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首页> 外文期刊>Cornea >Guinea pig models of acute keratitis responses.
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Guinea pig models of acute keratitis responses.

机译：豚鼠急性角膜炎反应模型。

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PURPOSE: Contact lens acute red eye (CLARE) and infiltrative keratitis (IK) are inflammatory responses of the eye associated with extended wear of soft contact lenses and are associated with bacterial colonization of contact lenses. Development of animal models to study these conditions is of importance in confirming risk factors and developing preventative strategies. METHODS: Silicone hydrogel contact lenses contaminated with a human CLARE isolate of Pseudomonas aeruginosa at inocula ranging from 1 x 10 to 6 x 10 colony-forming units (CFUs) for 15 minutes, 1 hour, or 24 hours were worn by guinea pigs for times up to 48 hours in 3 models designed to simulate contamination during wear and contact lens care regimens. Eyes were examined by slit-lamp biomicroscopy and histology to evaluate ocular responses and inflammation. RESULTS: Three different models for the effects of bacterial contamination on the etiology of CLARE/IK were developed and tested in the guinea pig eye. Bacterial contamination of contact lenses was confirmed to be a major risk factor for the development of CLARE/IK and a dose response shown. The pathological features of CLARE/IK in the guinea pig were virtually identical to those observed in human eyes. CONCLUSION: The models characterized here provide a useful tool for development of interventional and therapeutic strategies for these important ocular inflammatory conditions.
机译：目的：角膜接触镜急性红眼（CLARE）和浸润性角膜炎（IK）是与软性角膜接触镜长时间佩戴相关的眼睛炎症反应，并与角膜接触镜细菌定植有关。开发用于研究这些疾病的动物模型对于确认危险因素和制定预防策略至关重要。方法：豚鼠戴了几次被人类铜绿假单胞菌CLARE分离物污染的有机硅水凝胶隐形眼镜，接种时间为1 x 10至6 x 10个菌落形成单位（CFU），持续15分钟，1小时或24小时。 3个模型可长达48小时，可模拟佩戴和隐形眼镜护理过程中的污染。通过裂隙灯生物显微镜和组织学检查眼睛，以评估眼部反应和炎症。结果：在豚鼠眼中开发并测试了三种不同的细菌污染对CLARE / IK病因的影响模型。已确认隐形眼镜的细菌污染是CLARE / IK发生的主要危险因素，并显示了剂量反应。豚鼠CLARE / IK的病理特征与人眼观察到的几乎相同。结论：这里表征的模型为这些重要的眼部炎症性疾病的介入和治疗策略的开发提供了有用的工具。

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