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首页> 外文期刊>Cornea >Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits.
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Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits.

机译：羊膜作为基质培养角膜缘角膜上皮细胞用于兔自体移植。

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PURPOSE: To examine the viability of using human amniotic membrane as substrate for culturing corneal epithelial cells and transplanting them onto severely injured rabbit eyes. METHODS: An ocular-surface injury was created in the right eye of eight rabbits by a lamellar keratectomy extending 5 mm outside the limbus. Next, from the limbal region of the uninjured left eyes of five of these animals, a small biopsy of corneal epithelial cells was taken and cultured on acellular human amniotic membrane. One month later, the invading conjunctiva that covered the corneal surface of all eight injured eyes was surgically removed. Five of the eyes then received grafts of amniotic membrane containing autologous cultured epithelial cells, whereas the other three received grafts of acellular amniotic membrane alone. RESULTS: A confluent primary culture of limbal corneal epithelial cells was established on acellular human amniotic membrane after 14 days. Cells were partially stratified and fairly well attached to the underlying amniotic membrane, although a fully formed basement membrane was not evident. The three rabbits that received amniotic membrane transplantation alone all had total epithelial defects on the graft in the early postoperative period. Eyes that were grafted with amniotic membrane that contained cultivated epithelial cells, however, were all successfully epithelialized up to 5 days after surgery. CONCLUSION: Autologous transplantation of cultivated corneal epithelium is feasible by using acellular amniotic membrane as a carrier.
机译：目的：探讨使用人羊膜作为基质培养角膜上皮细胞并将其移植到严重受伤的兔子眼中的可行性。方法：通过在角膜缘外侧延伸5 mm的板层角膜切除术在八只兔子的右眼中造成眼表损伤。接下来，从其中五只动物的未受伤左眼的角膜缘区域，取一小片角膜上皮细胞活检并在无细胞人羊膜上培养。一个月后，手术切除覆盖了八只受伤的眼睛的角膜表面的侵袭性结膜。然后，五只眼接受含有自体培养的上皮细胞的羊膜移植物，而其他三只接受单独的无细胞羊膜移植物。结果：14天后，在脱细胞的人羊膜上建立了角膜缘角膜上皮细胞的融合原代培养物。尽管没有明显形成完整的基底膜，但细胞被部分分层并相当牢固地附着在下面的羊膜上。在术后早期，仅接受羊膜移植的三只兔子在移植物上都具有全部上皮缺损。但是，移植有包含培养的上皮细胞的羊膜的眼在手术后最多5天都成功上皮。结论：以脱细胞羊膜为载体，自体培养角膜上皮是可行的。

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