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首页> 外文期刊>Biochimica et biophysica acta. Molecular basis of disease: BBA >Self-regulation of the endothelin receptor system in choriocarcinoma cells
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Self-regulation of the endothelin receptor system in choriocarcinoma cells

机译：绒毛膜癌细胞中内皮素受体系统的自我调节

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摘要

The human trophoblast secretes endothelin-1 (ET-1) and expresses ET receptors. The present study tested whether the transformed BeWo, JAR and JEG-3 choriocarcinoma cells: (1) secrete endothelin-1 (ET-1); (2) express both ET-A and ET-B receptor subtypes; and (3) have the potential to allow for autologous regulation of ET-receptor proteins. The cells were cultured for 24/48 h with or without 10% FCS and, in experiments on receptor regulation, with ET-1 (5-20 nM and 10 μM). ET-1 secretion was measured by RIA and receptor levels by immunoblotting. All cell types secreted ET-1 albeit at different levels and sensitivity to FCS. All cell lines expressed both ET-A (JEG-3 >BeWo = JAR) and ET-B (JEG-3 = JAR > BeWo) receptor subtypes, which could be up- and downregulated depending on ET-1 concentration, culture time and FCS presence. It is concluded that BeWo, JAR and JEG-3 choriocarcinoma cells secrete ET-1 and express both ET-A and ET-B receptor subtypes. The receptor levels can be regulated by ET-1. This provides the molecular basis for an autocrine system with the potential of autologous regulation of yet unidentified ET-1-induced functions.
机译：人滋养细胞分泌内皮素-1（ET-1）并表达ET受体。本研究测试了转化后的BeWo，JAR和JEG-3绒毛膜癌细胞是否：（1）分泌内皮素-1（ET-1）; （2）同时表达ET-A和ET-B受体亚型; （3）有潜力允许自体调节ET受体蛋白。将细胞在有或没有10％FCS的情况下培养24/48小时，并在受体调节实验中以ET-1（5-20​​ nM和10μM）培养。 ET-1分泌通过RIA测定，受体水平通过免疫印迹测定。所有细胞类型都分泌ET-1，尽管其水平和对FCS的敏感性不同。所有细胞系均表达ET-A（JEG-3> BeWo = JAR）和ET-B（JEG-3 = JAR> BeWo）受体亚型，根据ET-1浓度，培养时间和FCS的存在。结论是BeWo，JAR和JEG-3绒毛膜癌细胞分泌ET-1并表达ET-A和ET-B受体亚型。受体水平可以由ET-1调节。这为自分泌系统提供了分子基础，并具有自发调控尚未确定的ET-1诱导功能的潜力。

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