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首页> 外文期刊>The Biochemical Journal >Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters
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Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters

机译：NarL依赖的激活和Fis依赖的抑制之间的竞争控制了大肠杆菌yeaR和ogt启动子的表达

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摘要

The Escherichia coli NarL protein is a global gene regulatory factor that activates transcription at many target promoters in response to nitrate and nitrite ions. Although most NarL-dependent promoters are also co-dependent on a second transcription factor, FNR protein, two targets, the yeaR and ogt promoters, are activated by NarL alone with no involvement of FNR. Biochemical and genetic studies presented here show that activation of the yeaR promoter is dependent on the binding of NarL to a single target centred at position -43.5, whereas activation at the ogt promoter requires NarL binding to tandem DNA targets centred at position -45.5 and -78.5. NarL-dependent activation at both the yeaR and ogt promoters is decreased in rich medium and this depends on Fis, a nucleoid-associated protein. DNase I footprinting studies identified Fis-binding sites that overlap the yeaR promoter NarL site at position -43.5, and the ogt promoter NarL site at position -78.5, and suggest that Fis represses both promoters by displacing NarL. The ogt gene encodes an O6-alkylguanine DNA alkyltransferase and, hence, this is the first report of expression of a DNA repair function being controlled by nitrate ions.
机译：大肠杆菌NarL蛋白是一种全球性的基因调节因子，可响应硝酸盐和亚硝酸盐离子激活许多靶标启动子的转录。尽管大多数NarL依赖性启动子也共同依赖于第二转录因子FNR蛋白，但两个靶标yeaR和ogt启动子却被NarL单独激活，而没有FNR参与。此处进行的生化和遗传研究表明，yeaR启动子的激活取决于NarL与以-43.5为中心的单个靶标的结合，而在ogt启动子的激活则需要NarL与以-45.5和-4为中心的串联DNA靶标结合。 78.5。在丰富的培养基中，yeaR和ogt启动子上的NarL依赖性激活都降低了，这取决于Fis（一种与核苷酸相关的蛋白）。 DNase I足迹研究确定了Fis结合位点，该位点在-43.5位的yeaR启动子NarL位点和在-78.5位的ogt启动子NarL位点重叠，并暗示Fis通过置换NarL抑制两个启动子。 ogt基因编码O6-烷基鸟嘌呤DNA烷基转移酶，因此，这是DNA修复功能受硝酸根离子控制表达的第一个报道。

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