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Induction of micromutation in M2 and its relationship for production of high yielding mutants in blackgram.

机译：黑变图中M 2 中微突变的诱导及其与高产突变体产生的关系。

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Seeds of two blackgram varieties PDU-1 and Sarala were treated with three doses each of Gamma-rays, EMS, NG, MH and combination. The M1 generation was harvested in bulk and the M2 to M4 generations were raised during 2003-04. In M2 generation, most treatment populations exhibited reduction in mean and increase in variance for all the six quantitative traits studied and magnitude of such change varied with mutagens, their doses and the variety. The negative shift of mean was more conspicuous for yield/plant in Basant Bahar and plant height in Sarala. In most cases, higher doses of mutagens induced greater variance. A comparative study of the effect of mutagens revealed that the shift of mean and variance was more pronounced in NG and combined treatment of both the varieties. The genetic parameters like range, mean, GCV, heritability and genetic advance in M2 populations were estimated for important yield components such as pods/plant, seeds/pod, 100-seed weight and yield/plant. Almost all mutagenic treatments induced wider range of variation than parent variety in both the directions, GCV estimates varied with mutagenic treatments and were moderately high for pods/plant and yield/plant in both the varieties. Heritability estimates for different traits were relatively higher magnitude indicating greater scope of selection, GA as percentage of mean also increased in treatments and comparatively higher for pods/plant in both the varieties, in this study, mutagenic treatments were classified into four groups on the basis of M2 mean and variance for yield/plant. Following selection among the M2 plants and M3 progenies on the basis of higher yield, 11 high yielding mutant cultures in PDU-1 and seven mutants in Sarala were isolated in M4 generation. Vast majority of the high yielding M3 progenies and M4 mutant cultures were from the groups of M2 mutagenic treatments showing significantly higher population variance for yield/plant. Thus, selection of high yielding M2 plants and M3 progenies in mutagenic treatments with much increased M2 population variance for yield would be effective in isolation of high yielding micromutant cultures.
机译：用伽玛射线，EMS，NG，MH和组合的三种剂量分别处理两个黑克勒姆品种PDU-1和Sarala的种子。在2003-04年期间，大批收获了M 1 世代，并繁殖了M 2 至M 4 世代。在M 2 世代中，大多数治疗种群对所研究的全部六个定量性状均表现出均值降低和方差增加，且这种变化的幅度随诱变剂，剂量和品种的不同而变化。在Basant Bahar，单产/植株和Sarala的株高对均值的负向变化更为明显。在大多数情况下，更高剂量的诱变剂会引起更大的差异。对诱变剂作用的比较研究表明，均值和方差的变化在NG和两个品种的联合处理中更为明显。估计了M 2 种群的遗传参数，例如范围，平均值，GCV，遗传力和遗传进展，这些重要参数包括荚果/植物，种子/荚果，100粒重和产量/植物等重要产量成分。几乎所有诱变处理都在两个方向上引起了比亲本品种更宽的变异范围，GCV估计值随诱变处理而变化，并且两个品种的豆荚/植物和产量/植物均中等偏高。不同性状的遗传力估计值相对较高，表明选择范围更大，两种品种的处理中GA的均值百分比也均增加，豆荚/植物的相对性也较高，在本研究中，诱变处理方法在此基础上分为四组 2 的平均值和方差对产量/植物的影响。在以较高产量为基础从M 2 植物和M 3 后代中进行选择后，在M中分离了PDU-1中的11种高产突变体培养物和Sarala中的7个突变体 4 代。绝大多数高产的M 3 后代和M 4 突变体培养物来自M 2 诱变处理组，显示出较高的种群变异。产量/植物。因此，在诱变处理中选择高产的M 2 植物和M 3 后代，使诱变处理中的M 2 群体变异大大增加，这将是有效的。高产微突变培养物的分离。

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