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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >The crucial role of chromogranins in storage and exocytosis revealed using chromaffin cells from chromogranin A null mouse.
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The crucial role of chromogranins in storage and exocytosis revealed using chromaffin cells from chromogranin A null mouse.

机译：嗜铬粒蛋白在贮藏和胞吐作用中的关键作用是利用嗜铬粒蛋白A null小鼠的嗜铬细胞产生的。

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摘要

Chromogranins (Cgs) are the major soluble proteins of dense-core secretory vesicles. Chromaffin cells from Chga null mice [chromogranin A knock-out (CgA-KO)] exhibited approximately 30% reduction in the content and in the release of catecholamines compared with wild type. This was because of a lower secretion per single exocytotic event, rather than to a lower frequency of exocytotic events. Cell incubation with L-DOPA produced an increase in the vesicular amine content of wild-type, but not CgA-KO vesicles. In contrast, intracellular electrochemistry showed that L-DOPA produced a significantly larger increase in cytosolic amines in CgA-KO cells than in the wild type. These data indicate that the mechanisms for vesicular accumulation in CgA-KO cells were fully saturated. Patch-amperometry recordings showed a delayed initiation of the amperometric signal after vesicle fusion, whereas no changes were observed in vesicle size or fusion pore kinetics despite the smaller amine content. We conclude that intravesicular proteins are highly efficient systems directly implicated in transmitter accumulation and in the control of neurosecretion.
机译：嗜铬粒蛋白（Cgs）是致密核心分泌囊泡的主要可溶性蛋白。与野生型相比，来自Chga缺失小鼠的嗜铬细胞[chromogranin A敲除（CgA-KO）]的儿茶酚胺含量和释放减少了约30％。这是因为每个单个胞吐事件的分泌量较低，而不是由于胞吐事件的频率较低。用L-DOPA进行细胞孵育会增加野生型囊泡胺的含量，但不会增加CgA-KO囊泡的含量。相反，细胞内电化学分析表明，与野生型相比，CgA-KO细胞中L-DOPA产生的胞质胺增加量明显更大。这些数据表明，CgA-KO细胞中囊泡积累的机制已完全饱和。膜片安培法记录显示，在囊泡融合后，安培计信号的延迟启动，尽管胺含量较小，但囊泡大小或融合孔动力学没有观察到变化。我们得出的结论是，囊泡内蛋白质是高效系统，直接参与递质的积累和神经分泌的控制。

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