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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Environment-Responsive Fluorescent Nucleoside Analogue Probe for Studying Oligonucleotide Dynamics in a Model Cell-like Compartment
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Environment-Responsive Fluorescent Nucleoside Analogue Probe for Studying Oligonucleotide Dynamics in a Model Cell-like Compartment

机译：环境响应型荧光核苷模拟探针，用于研究模型细胞状隔室中的寡核苷酸动力学。

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摘要

The majority of fluorescent nucleoside analogue probes that have been used in the in vitro study of nucleic acids are not suitable for cell-based biophysical assays because they exhibit excitation maxima in the UV region and low quantum yields within oligonucleotides. Therefore, we propose that the photophysical characterization of oligonucleotides labeled with a fluorescent nucleoside analogue in reverse micelles (RM), which are good biological membrane models and UV-transparent, could provide an alternative approach to studying the properties of nucleic acids in a celllike confined environment. In this context, we describe the photophysical properties of an environment-sensitive fluorescent uridine analogue (l), based on the 5-(benzo[b]thiophen-2~ yl)pyrimidine core, in micelles and RM. The emissive nucleoside, which is polarity-and viscosity-sensitive, reports the environment of the surfactant assemblies via changes in its fluorescence properties. The nucleoside analogue, incorporated into an RNA oligonucleotide and hybridized to its complementary DNA and RNA oligonucleotides, exhibits a significantly higher fluorescence intensity, lifetime, and anisotropy in RM than in aqueous buffer, which is consistent with the environment of RM. Collectively, our results demonstrate that nucleoside 1 could be utilized as a fluorescent label to study the function of nucleic acids in a model cellular milieu.
机译：在核酸的体外研究中使用的大多数荧光核苷类似物探针不适合基于细胞的生物物理分析，因为它们在UV区显示出极大的激发，并且在寡核苷酸内显示出低的量子产率。因此，我们认为反向胶束（RM）中标记有荧光核苷类似物的寡核苷酸的光物理特征是良好的生物膜模型和紫外线透明性，可以为研究细胞状密闭核酸的特性提供另一种方法。环境。在这种情况下，我们描述了基于环境的5-（苯并[b]噻吩-2-基）嘧啶核的环境敏感型荧光尿苷类似物（l）在胶束和RM中的光物理性质。极性和粘度敏感的发射核苷通过其荧光性质的变化报告表面活性剂组件的环境。掺入RNA寡核苷酸并与其互补的DNA和RNA寡核苷酸杂交的核苷类似物，在RM中的荧光强度，寿命和各向异性明显高于在水性缓冲液中，这与RM的环境一致。总的来说，我们的结果表明核苷1可以用作荧光标记来研究核酸在模型细胞环境中的功能。

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