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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Evaluation of Diffusion Coefficient of Thiocholine in Enzyme-Loaded Polypyrrole Composite Film through Different Methods and Electrode Polarization
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Evaluation of Diffusion Coefficient of Thiocholine in Enzyme-Loaded Polypyrrole Composite Film through Different Methods and Electrode Polarization

机译：通过不同方法和电极极化评估酶负载的聚吡咯复合膜中胆碱的扩散系数

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摘要

In this work, the diffusion of thiocholine ion into an enzyme-loaded polypyrrole film was evaluated by different methods, and the results were compared to identify the most suitable method. The enzyme-loaded polypyrrole film was coated with a thin layer of gelatin and gluteraldehyde so as to prevent enzyme leaching. Diffusion coefficients under normal and prepolarized conditions were calculated by five different methods, namely, the Cottrell method, the method of Peerce and Bard, the theoretical impedance model, the electrochemically stimulated conformational relaxation (ESCR) method, and the direct impedance measurement method. The theoretical model of Vortynstev was used to calculate the parameters from the impedance spectra using simplex technique in MATLAB. The results indicate that under normal unpolarized condition the ESCR method gives a diffusion coefficient close to that given by Vortynstev method, but under polarized conditions the Cottrell method can provide a better value of diffusion coefficient than ESCR. The diffusion coefficient of thiocholine in PPy composite film from an electrolytic background of phosphate buffer of pH 7.4 was found to be 1.00 x 10(-8) cm(-2) s(-1) based on Vortynstev method. The mechanism of thiocholine diffusion into the positively charged/polarized matrix is attributed to be through the formation of a dinegative ion between thiocholine and phosphate anion via electrostatic attraction.
机译：在这项工作中，通过不同的方法评估了硫代胆碱离子在负载酶的聚吡咯膜中的扩散，并比较了结果以找出最合适的方法。在酶负载的聚吡咯膜上涂上一层明胶和戊二醛薄层，以防止酶浸出。通过五种不同的方法计算正常和预极化条件下的扩散系数，即Cottrell方法，Peerce和Bard方法，理论阻抗模型，电化学刺激的构象松弛（ESCR）方法和直接阻抗测量方法。使用MATLAB中的单纯形法，使用Vortynstev的理论模型从阻抗谱中计算参数。结果表明，在正常的非极化条件下，ESCR方法的扩散系数接近于Vortynstev法给出的扩散系数，但是在极化条件下，Cottrell方法可以提供比ESCR更好的扩散系数值。根据Vortynstev方法，从pH 7.4的磷酸盐缓冲液的电解本底来看，硫代胆碱在PPy复合膜中的扩散系数为1.00 x 10（-8）cm（-2）s（-1）。硫代胆碱扩散到带正电/极化的基质中的机理归因于通过静电吸引在硫代胆碱和磷酸根阴离子之间形成负离子。

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