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首页> 外文期刊>Cryobiology: International Journal of Low Temperature Biology and Medicine >Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm
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Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm

机译：激素植入对大西洋大比目鱼（Hippoglossus hippoglossus L.）精子冷冻保存的影响

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摘要

Hormone implantation is widely applied in halibut (Hippoglossus hippoglossus L.) aquaculture to extend the sperm production season of broodstock males. The ability to combine this technique with cryopreservation would increase sperm availability, thereby improving reproduction success and facilitating gene management. In this paper, the cryopreservation ability of sperm from hormone-treated males was examined at three times post-implantation and compared with that of sperm from males that were not hormone-treated. All sperm samples were cryopreserved using the same method. The effectiveness of these techniques was assessed by examining the fertilization rate and motility of thawed sperm. The spermotocrit and concentration of fresh sperm samples were measured to reveal the effect of hormone implantation on sperm characteristics. The reported results indicate that hormone implantation did not affect cryopreservation efficiency. The fertilization rate resulting from thawed sperm of hormone-treated males showed no significant difference from that of untreated males or from fresh sperm. A significant positive relationship was demonstrated between the spermatocrit and concentration of sperm; and a significant decrease of spermatocrit was found in sperm collected from hormone-treated males 14. days post-implantation. No significant linear relationship between spermotocrit and fertilization rate of thawed sperm was shown.
机译：激素植入被广泛应用于大比目鱼（Hippoglossus hippoglossus L.）的水产养殖中，以延长亲鱼雄性的精子生产季节。将该技术与冷冻保存相结合的能力将提高精子的利用率，从而提高繁殖成功率并促进基因管理。在本文中，对荷尔蒙处理过的雄性精子的冷冻保存能力在植入后三遍进行了检查，并将其与未经荷尔蒙处理过的雄性精子进行了比较。使用相同的方法将所有精子样品冷冻保存。通过检查解冻精子的受精率和运动力来评估这些技术的有效性。测量精子压积和新鲜精子样品的浓度，以揭示激素植入对精子特征的影响。报道的结果表明激素植入并没有影响冷冻保存效率。经激素处理的雄性精子解冻后的受精率与未经处理的雄性或新鲜精子无显着差异。精细胞压积与精子浓度之间存在显着的正相关。植入后14天，从接受激素治疗的男性收集的精子中发现精子比容显着下降。精子压积与解冻精子的受精率之间没有显着的线性关系。

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