...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >An alternative renewable source of squalene for use in emulsion adjuvants
【24h】

An alternative renewable source of squalene for use in emulsion adjuvants

机译：用于乳液佐剂的角鲨烯的替代可再生来源

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Emulsions have been used to boost immunogenicity of antigens since the discovery of complete Freunds adjuvant. Optimization to reduce reactogenicity of emulsion adjuvants lead to the development of oil in water emulsions based on squalene. MF59 is an oil-in-water emulsion that is a component of an approved influenza product in Europe. Currently MF59 is manufactured from squalene derived from an animal source. Recently a high purity plant-derived squalene source has become available at an appropriate purity for a vaccine adjuvant. The purpose of this study was to evaluate and compare animal-derived squalene and plant-derived squalene for equivalency. Nanoemulsions were prepared and analyzed for size and viscosity prepared from each source. The two emulsions were administered in two separate animal studies, one focusing on Neisseria meningitidis B, and one focusing on influenza. Readouts were ELISA titers for each antigen and serum bactericidal activity for N. meningitidis B, and hemagglutinin inhibition for influenza to see the functionality of the antibodies produced. Results indicate that there are no differences between the antibodies elicited after immunization from an emulsion made with oil derived from either an animal or plant-source
机译：自从发现完整的弗氏佐剂以来，乳剂已用于增强抗原的免疫原性。降低乳化助剂反应原性的优化导致了基于角鲨烯的水包油型乳化剂的开发。 MF59是一种水包油乳剂，是欧洲认可的流感产品的组成部分。目前，MF59是由动物来源的角鲨烯制成的。最近，高纯度植物来源的角鲨烯来源已经以合适的纯度用于疫苗佐剂。本研究的目的是评估和比较动物源角鲨烯和植物源角鲨烯的等效性。制备纳米乳液，并分析由每种来源制备的尺寸和粘度。两种乳液分别在两项动物实验中给药，其中一项针对脑膜炎奈瑟氏球菌B，另一项针对流感。读数是每种抗原的ELISA效价和脑膜炎双球菌B的血清杀菌活性，以及​​流感的血凝素抑制作用，以查看所产生抗体的功能。结果表明，用动物或植物来源的油制成的乳状液进行免疫接种后所引发的抗体之间没有差异

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号