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首页> 外文期刊>Vaccine >The use of green fluorescent protein as a marker for Brucella vaccines.
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The use of green fluorescent protein as a marker for Brucella vaccines.

机译：绿色荧光蛋白作为布鲁氏菌疫苗的标记物的用途。

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Brucellosis is an important malady of productive and wildlife animals and a worldwide zoonosis. The use of effective vaccines and the corresponding diagnostic tests that allow differentiating infected from vaccinated animals are essential tools to control the disease. For this, a prototype of Brucella abortus S19 vaccine expressing green fluorescent protein (S19-GFP) was constructed. The S19-GFP was readily identified under ultraviolet light by macroscopic and microscopic examination and maintained all the biochemical characteristics of the parental S19 vaccine. S19-GFP replicated ex vivo and in vivo, and protected mice against challenge with virulent B. abortus to the same extent as the isogenic S19. An immunoenzymatic assay designed to measure anti-GFP antibodies allowed the discrimination between mice vaccinated with S19-GFP and those immunized with S19. Both vaccines raised antibodies against lipopolysaccharide molecule to similar levels. This experimental model constitutes a "proof of concept" for the use of Brucella-GFP vaccines and associated diagnostic tests to distinguish vaccinated from naturally Brucella infected animals.Digital Object Identifier http://dx.doi.org/10.1016/j.vaccine.2010.09.109
机译：布鲁氏菌病是生产性和野生动物的重要疾病，也是全世界的人畜共患病。有效疫苗的使用和相应的诊断测试，可以区分感染动物和疫苗接种动物，是控制疾病的重要工具。为此，构建了表达绿色荧光蛋白（S19-GFP）的流产布鲁氏菌S19疫苗的原型。 S19-GFP很容易在紫外线下通过宏观和微观检查鉴定，并保持了亲本S19疫苗的所有生化特性。 S19-GFP可在体内和体内复制，并保护小鼠免受强毒 B的攻击。流产的程度与同基因S19相同。通过设计用于测量抗GFP抗体的免疫酶分析，可以区分接种S19-GFP的小鼠和接种S19的小鼠。两种疫苗都将针对脂多糖分子的抗体提高到相似的水平。该实验模型构成了使用布鲁氏菌 -GFP疫苗和相关诊断测试来区分接种的疫苗和天然布鲁氏菌动物的“概念验证”。DigitalObject Identifier http： //dx.doi.org/10.1016/j.vaccine.2010.09.109

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