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首页> 外文期刊>Vaccine >alpha 1-giardin based live heterologous vaccine protects against Giardia lamblia infection in a murine model.
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alpha 1-giardin based live heterologous vaccine protects against Giardia lamblia infection in a murine model.

机译：基于alpha 1-giardin的活异源疫苗可在鼠模型中预防 Giardia lamblia 感染。

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Giardia lamblia is a leading protozoan cause of diarrheal disease worldwide, yet preventive medical strategies are not available. A crude veterinary vaccine has been licensed for cats and dogs, but no defined human vaccine is available. We tested the vaccine potential of three conserved antigens previously identified in human and murine giardiasis, alpha 1-giardin, alpha -enolase, and ornithine carbamoyl transferase, in a murine model of G. lamblia infection. Live recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine strains were constructed that stably expressed each antigen, maintained colonization capacity, and sustained total attenuation in the host. Oral administration of the vaccine strains induced antigen-specific serum IgG, particularly IgG2A, and mucosal IgA for alpha 1-giardin and alpha -enolase, but not for ornithine carbamoyl transferase. Immunization with the alpha 1-giardin vaccine induced significant protection against subsequent G. lamblia challenge, which was further enhanced by boosting with cholera toxin or sublingual alpha 1-giardin administration. The alpha -enolase vaccine afforded no protection. Analysis of alpha 1-giardin from divergent assemblage A and B isolates of G. lamblia revealed >97% amino acid sequence conservation and immunological cross-reactivity, further supporting the potential utility of this antigen in vaccine development. Together. These results indicate that alpha 1-giardin is a suitable candidate antigen for a vaccine against giardiasis.
机译：贾第虫（Giardia lamblia）是全世界腹泻病的主要原生动物病因，但尚无预防性医学策略。粗制兽用疫苗已获准用于猫和狗，但尚无明确的人用疫苗。我们在G鼠模型中测试了先前在人和鼠的贾第鞭毛虫病中鉴定出的三种保守抗原，α1-贾第蛋白，α-烯醇酶和鸟氨酸氨基甲酰基转移酶的疫苗潜力。兰比亚感染。构建了活的重组减毒小肠沙门氏菌鼠伤寒沙门氏菌疫苗株，该菌株在宿主中稳定表达每种抗原，保持定殖能力并持续减毒。口服该疫苗株可诱导产生抗原特异性血清IgG，特别是IgG 2A 和粘膜IgA，从而产生α1-贾第蛋白和α-烯醇酶，但鸟氨酸氨基甲酰基转移酶则不能。用α1-giardin疫苗免疫诱导了对随后的G的显着保护。羔羊挑战，可通过霍乱毒素或舌下α1-giardin的施用进一步加强。 α-烯醇酶疫苗没有提供任何保护。分析来自G的不同组合A和B分离株的α1-giardin。 Lamblia 揭示了> 97％的氨基酸序列保守性和免疫学交叉反应性，进一步支持了该抗原在疫苗开发中的潜在用途。一起。这些结果表明α1-贾第蛋白是抗贾第鞭毛虫病疫苗的合适候选抗原。

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