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首页> 外文期刊>Vaccine >Vaccine adjuvants alum and MF59 induce rapid recruitment of neutrophils and monocytes that participate in antigen transport to draining lymph nodes.
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Vaccine adjuvants alum and MF59 induce rapid recruitment of neutrophils and monocytes that participate in antigen transport to draining lymph nodes.

机译：疫苗佐剂明矾和MF59诱导嗜中性粒细胞和单核细胞快速募集，这些细胞参与抗原转运至引流淋巴结。

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Vaccine adjuvants such as alum and the oil-in-water emulsion MF59 are used to enhance immune responses towards pure soluble antigens, but their mechanism of action is still largely unclear. Since most adjuvanted vaccines are administered intramuscularly, we studied immune responses in the mouse muscle and found that both adjuvants were potent inducers of chemokine production and promoted rapid recruitment of CD11b+ cells. The earliest and most abundantly recruited cell type are neutrophils, followed by monocytes, eosinophils and later dendritic cells (DCs) and macrophages. Using fluorescent forms of MF59 and ovalbumin (OVA) antigen, we show that all recruited cell types take up both adjuvant and antigen to transport them to the draining lymph nodes (LNs). There, we found antigen-positive neutrophils and monocytes within hours of injection, later followed by B cells and DCs. Compared to alum, MF59-injection lead to a more prominent neutrophil recruitment and a more efficient antigen re-localization from the injection site to the LN. As antigen-transporting neutrophils were observed in draining LNs, we asked whether these cells play an essential role in MF59-mediated adjuvanticity. However, antibody-mediated neutrophil ablation left MF59-adjuvanticity unaltered. Further studies will reveal whether other single cell types are crucial or whether the different recruited cell populations are redundant with overlapping functions.
机译：疫苗佐剂如明矾和水包油乳剂MF59用于增强对纯可溶性抗原的免疫反应，但其作用机理仍不清楚。由于大多数佐剂疫苗是通过肌肉注射给药的，因此我们研究了小鼠肌肉中的免疫反应，发现这两种佐剂都是趋化因子产生的有效诱导剂，并促进了CD11b + 细胞的快速募集。最早和募集最多的细胞是嗜中性粒细胞，其次是单核细胞，嗜酸性粒细胞和后来的树突状细胞（DC）和巨噬细胞。使用荧光形式的MF59和卵清蛋白（OVA）抗原，我们显示所有募集的细胞类型都吸收佐剂和抗原，将其转运至引流淋巴结（LNs）。在那里，我们在注射后数小时内发现了抗原阳性的中性粒细胞和单核细胞，随后是B细胞和DC。与明矾相比，MF59注射导致更显着的嗜中性白细胞募集和更有效的抗原从注射部位到LN的重新定位。由于在引流LNs中观察到抗原转运性中性粒细胞，我们询问这些细胞是否在MF59介导的佐剂中起重要作用。然而，抗体介导的嗜中性粒细胞消融使MF59-佐剂性保持不变。进一步的研究将揭示其他单个细胞类型是否至关重要，或者不同募集的细胞群体是否具有重叠功能是多余的。

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