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首页> 外文期刊>Vaccine >Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development.
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Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development.

机译：天然和重组赖氨酸脱羧酶免疫对犬齿龈炎发展的影响。

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Periodontal disease, gingival inflammation (gingivitis) and periodontal attachment loss (periodontitis), causes tooth loss and susceptibility to chronic inflammation. Professionally scaling and cleaning the teeth regularly controls the disease, but is expensive in companion animals. Eikenella corrodens is common in canine oral cavities where it is a source of lysine decarboxylase (LDC). In human dental biofilms (plaques), LDC converts lysine to cadaverine and impairs the gingival epithelial barrier to bacteria. LDC vaccination may therefore retard gingivitis development. Year-old beagle dogs provided blood samples, and had weight and clinical measurements (biofilm and gingivitis) recorded. After scaling and cleaning, two dogs were immunized subcutaneously with 0.2 mg native LDC from E. corrodens and 2 sets of four dogs with 0.2 mg recombinant LDC purified from Escherichia coli. A third set of 4 dogs was immunized intranasally. RehydragelReg., EmulsigenReg., PolygenTM or CarbigenTM were used as adjuvant. Four additional pairs of dogs were sham-immunized with each adjuvant alone (controls). Immunizations were repeated twice, 3 weeks apart, and clinical measurements were obtained after another 2 weeks, when the teeth were scaled and cleaned again. Tooth brushing was then stopped and the diet was changed from hard to soft chow. Clinical measurements were repeated after 1, 2, 3, 4, 6 and 8 weeks. Compared with sham-immunized dogs, gingivitis was reduced over all 8 weeks of soft diet after subcutaneous immunization with native LDC, or after intranasal immunization with recombinant LDC in CarbigenTM, but for only 6 of the 8 weeks after subcutaneous immunization with recombinant LDC in EmulsigenReg. (repeated measures ANOVA). Subcutaneous vaccination induced a strong serum IgG antibody response that decreased during the soft diet period, whereas intranasal immunization induced a weak serum IgA antibody response that did not decrease. Immunization with recombinant LDC may provide protection from gingivitis if procedures are optimized.
机译：牙周疾病，牙龈发炎（牙龈炎）和牙周附着丧失（牙周炎）会导致牙齿脱落和对慢性炎症的敏感性。专业地洗牙和清洁牙齿可以定期控制疾病，但是在伴侣动物中价格昂贵。腐蚀的艾肯氏菌在犬口腔中很常见，在赖氨酸中赖氨酸是脱羧酶（LDC）的来源。在人类牙齿生物膜（斑块）中，LDC将赖氨酸转化为尸胺，并损害了牙龈上皮对细菌的屏障。因此，最不发达国家疫苗接种可能会延迟牙龈炎的发展。一岁的比格犬提供血液样本，并记录体重和临床测量值（生物膜和牙龈炎）。洗牙和清洁后，用0.2 mg天然大肠杆菌的天然LDC皮下免疫两只狗，用0.2 mg从大肠杆菌中纯化的重组LDC皮下免疫接种两组四只狗。第三组4只狗经鼻内免疫。使用RehydragelReg。，EmulsigenReg。，Polygen TM 或Carbigen TM 作为佐剂。用另外的每种佐剂对另外四对狗进行假免疫（对照）。重复两次免疫，间隔3周，然后在再次洁牙并再次清洁牙齿后的2周后再进行临床测量。然后停止刷牙，饮食从硬食物改为软食物。在1、2、3、4、6和8周后重复临床测量。与假免疫的狗相比，在天然的LDC皮下免疫后，或在Carbigen 中的重组LDC鼻内免疫后，在软饮食的所有8周中牙龈炎都减少了，但仅在8周中有6周减少了在EmulsigenReg中用重组LDC皮下免疫后（重复测量方差分析）。皮下接种疫苗诱导了强烈的血清IgG抗体应答，该应答在软饮食期间降低了，而鼻内免疫接种引起的血清IgA抗体应答却没有降低。如果程序得到优化，重组LDC的免疫接种可提供免受牙龈炎的保护。

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