...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells.
【24h】

In vitro analysis of the factors contributing to the antiviral state induced by a plasmid encoding the viral haemorrhagic septicaemia virus glycoprotein G in transfected trout cells.

机译：体外分析转染鳟鱼细胞中编码病毒性败血病病毒糖蛋白G的质粒诱导的抗病毒状态的因素。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have found out that transfection of the RTG-2 cell line with the viral haemorrhagic septicaemia virus (VHSV) glycoprotein G (GVHSV)-coding plasmid induces an anti-VHSV state, similar to that induced by poly I:C. Taking the advantage of the constitutive expression of toll-like receptor 9 gene (tlr9) in RTG-2 cells, we have investigated whether this antiviral state was induced by the cytosine-phosphodiester-guanine (CpG) motifs present in the plasmid DNA, by the endogenous expression of GVHSV protein or by both elements. For that, we have analysed the expression profile of the rainbow trout tlr9 and several genes related to TLR9-mediated immune response in the absence or presence of a lysosomotropic drug that specifically blocks TLR9-CpG DNA interaction. The results suggested that the high levels of cell protection conferred by a plasmid encoding GVHSV gene are due to GVHSV rather than to the CpG motifs within plasmid DNA. Therefore, plasmid DNA might not play a key role in the immune response elicited by DNA vaccines or perhaps other receptors instead TLR9 could be implicated in CpG motifs recognition and signalling. In addition, since RTG-2 cells express tlr9 gene, this cell line could be a good tool for screening TLR9 agonists, such as the immunomodulatory oligonucleotides (IMOs), as fish DNA vaccine adjuvants.
机译：我们发现，用病毒性败血病病毒（VHSV）糖蛋白G（G VHSV ）编码质粒转染RTG-2细胞系可诱导抗VHSV状态，类似于由聚I：C。利用Toll样受体9基因（ tlr9 ）在RTG-2细胞中的组成型表达优势，我们研究了这种抗病毒状态是否由胞嘧啶-磷酸二酯-鸟嘌呤（CpG）基序诱导通过G VHSV 蛋白质的内源表达或两种元素共同存在于质粒DNA中。为此，我们分析了虹鳟鱼 tlr9 和几种与TLR9介导的免疫反应相关的基因的表达谱，这些基因在不存在或存在能特异性阻断TLR9-CpG DNA相互作用的溶致同性药物的情况下。结果表明，编码G VHSV 基因的质粒赋予的高水平的细胞保护作用是由于G VHSV 而不是质粒DNA中的CpG基序。因此，质粒DNA可能不会在DNA疫苗或其他受体引起的免疫应答中发挥关键作用，相反TLR9可能与CpG基序识别和信号传导有关。此外，由于RTG-2细胞表达 tlr9 基因，因此该细胞系可能是筛选TLR9激动剂（如免疫调节性寡核苷酸（IMOs））作为鱼类DNA疫苗佐剂的良好工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号