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首页> 外文期刊>Vaccine >Comparison of the immune responses in BALB/c mice following immunization with DNA-based and live attenuated vaccines delivered via different routes
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Comparison of the immune responses in BALB/c mice following immunization with DNA-based and live attenuated vaccines delivered via different routes

机译：比较通过不同途径递送的基于DNA的和减毒活疫苗免疫BALB / c小鼠后的免疫应答

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The objective of this study was to compare immune responses induced in BALB/c mice following immunization with pcDNA-GPV-VP2 DNA by gene gun bombardment (6 mu g) or by intramuscular (im) injection (100 mu g) with the responses to live attenuated vaccine by im injection (100 mu l). pcDNA3.1 (+) and physiological saline were used as controls. Peripheral blood samples were collected at 3, 7, 14, 21, 28, 35, 49, 63, 77 and 105 d after immunization. T lymphocyte proliferation was analyzed by KIT assay and enumeration of CD4(+), and CD8(+) T cell populations in peripheral blood was performed by flow cytometric analysis. Indirect ELISA was used to detect IgG levels. Cellular and humoral responses were induced by pcDNA-GPV-VP2 DNA and live virus vaccines. No differences were observed in T cell proliferation and CD8(+) T cell responses induced by the genetic vaccine regardless of the route of delivery. However, CD4(+) T cell responses and humoral immunity were enhanced in following gene gun immunization compared with im injection of the genetic vaccine. Cellular and humoral immunity was enhanced in following gene gun delivery of the genetic vaccine compared with the live attenuated vaccine. In conclusion, the pcDNA-GPV-VP2 DNA vaccine induced enhanced cellular and humoral immunity compared with that induced by the live attenuated vaccine
机译：这项研究的目的是比较通过基因枪轰击（6μg）或肌内（im）注射（100μg）用pcDNA-GPV-VP2 DNA免疫BALB / c小鼠后诱导的免疫应答与即时注射减毒活疫苗（100 ul）。将pcDNA3.1（+）和生理盐水用作对照。免疫后第3、7、14、21、28、35、49、63、77和105 d采集外周血样品。通过KIT分析法分析T淋巴细胞的增殖，并计数CD4（+），并通过流式细胞术分析外周血中的CD8（+）T细胞群体。间接ELISA用于检测IgG水平。 pcDNA-GPV-VP2 DNA和活病毒疫苗诱导了细胞和体液反应。不论传递途径如何，均未观察到遗传疫苗诱导的T细胞增殖和CD8（+）T细胞反应的差异。但是，与基因疫苗的即时注射相比，在随后的基因枪免疫中，CD4（+）T细胞反应和体液免疫得到了增强。与减毒活疫苗相比，基因疫苗在随后的基因枪递送后，细胞和体液免疫得到增强。总之，与减毒活疫苗相比，pcDNA-GPV-VP2 DNA疫苗诱导的细胞和体液免疫增强

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