A novel baculovirus vector shows efficient gene delivery of modified porcine reproductive and respiratory syndrome virus antigens and elicits specific immune response.

Karuppannan A. K.; Qiang Jia; Chang C. C.; Kwang J.

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A novel baculovirus vector shows efficient gene delivery of modified porcine reproductive and respiratory syndrome virus antigens and elicits specific immune response.

机译：一种新型杆状病毒载体显示出经修饰的猪繁殖与呼吸综合征病毒抗原的有效基因传递，并引发了特异性免疫反应。

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Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating epizootic of porcine species. Current vaccines are inadequate to control the disease burden and outbreaks in the field. We report a novel baculovirus vaccine vector with White spot syndrome virus immediate early 1 shuttle promoter, with strong activity in both insect cells and mammalian cells, for immunization against PRRSV. The insect cell cultured baculovirus vector produces PRRSV envelope glycoproteins ORF2a, ORF3, ORF4 and ORF5, which are similar to the antigens in the infectious PRRS virion, and these antigens are stably incorporated on the surface of the baculovirus. Further, the baculovirus vector efficiently transduces these antigens in cells of porcine origin, thereby simulating a live infection. The baculovirus vectored PRRSV antigens, upon inoculation in mice, elicits robust neutralizing antibodies against the infective PRRS virus. Further, the experiments indicate that hitherto under emphasized ORF2a and ORF4 are important target antigens for neutralizing PRRSV infectivity.Digital Object Identifier http://dx.doi.org/10.1016/j.vaccine.2013.08.101

机译：猪繁殖与呼吸综合症（PRRS）是对猪种造成经济破坏的动物流行病。当前的疫苗不足以控制现场的疾病负担和爆发。我们报告了一种新型杆状病毒疫苗载体，具有白斑综合症病毒立即早期1穿梭启动子，在昆虫细胞和哺乳动物细胞中均具有较强的活性，可针对PRRSV进行免疫。昆虫细胞培养的杆状病毒载体产生PRRSV包膜糖蛋白ORF2a，ORF3，ORF4和ORF5，与感染性PRRS病毒体中的抗原相似，并且这些抗原稳定地掺入杆状病毒的表面。此外，杆状病毒载体在猪来源的细胞中有效地转导这些抗原，从而模拟了活感染。杆状病毒载体的PRRSV抗原在小鼠中接种后，会引发针对感染性PRRS病毒的强大的中和抗体。此外，实验表明迄今为止强调的ORF2a和ORF4是中和PRRSV感染力的重要靶抗原。数字对象标识符http://dx.doi.org/10.1016/j.vaccine.2013.08.101

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《Vaccine》 |2013年第46期|共8页
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Karuppannan A. K.; Qiang Jia; Chang C. C.; Kwang J.;
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Porcine reproductive and respiratory syndrome virus; Baculovirus; Vaccine;

机译：猪繁殖与呼吸综合征病毒;杆状病毒;疫苗;

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1. A novel baculovirus vector shows efficient gene delivery of modified porcine reproductive and respiratory syndrome virus antigens and elicits specific immune response. [J] . Karuppannan A. K., Qiang Jia, Chang C. C., Vaccine . 2013,第46期

机译：一种新型杆状病毒载体显示出经修饰的猪繁殖与呼吸综合征病毒抗原的有效基因传递，并引发了特异性免疫反应。
2. Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV). [J] . Zheng Q, Chen D, Li P, Virus Genes . 2007,第3期

机译：在基于重组修饰牛痘病毒安卡拉（rMVA）的疫苗载体中，在不同启动子下共表达GP5和M蛋白增强了猪繁殖与呼吸综合征病毒（PRRSV）的体液和细胞免疫应答。
3. Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) [J] . Qisheng Zheng, Desheng Chen, Peng Li, Virus Genes . 2007,第3期

机译：在基于重组牛痘病毒安卡拉（rMVA）的疫苗载体中不同启动子下共表达GP5和M蛋白增强了猪繁殖与呼吸综合征病毒（PRRSV）的体液和细胞免疫应答
4. Porcine circovirus (PCV) as delivery virus vector to express porcine reproductive and respiratory syndrome virus (PRRSV) epitopes [C] . P. E. Piiieyro, S. P. Kenney, L. G. Gimenez-Lirola, Annual^Meeting of the American^Association^of^Swine^Veterinarians. . 2016

机译：猪胃肠病毒（PCV）作为递送病毒载体表达猪生殖和呼吸综合征病毒（PRRSV）表位
5. Investigation of immune response and protection induced by a combined vaccination of modified live and killed porcine reproductive and respiratory syndrome virus vaccines and its potential use in a swine herd. [D] . Nilubol, Dachrit. 2004

机译：联合接种改性活猪和灭活猪繁殖与呼吸综合症病毒疫苗的疫苗诱导的免疫应答和保护作用的研究及其在猪群中的潜在用途。
6. Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) [O] . Qisheng Zheng, Desheng Chen, Peng Li, -1

机译：在基于重组牛痘病毒安卡拉（rMVA）的疫苗载体中不同启动子下共表达GP5和M蛋白增强了猪繁殖与呼吸综合征病毒（PRRSV）的体液和细胞免疫应答
7. Evaluation of the use of non-pathogenic porcine circovirus type 1 as a vaccine delivery virus vector to express antigenic epitopes of porcine reproductive and respiratory syndrome virus [O] . Piñeyro, Pablo E, Kenney, Scott P, Giménez-Lirola, Luis G, 2016

机译：评价使用非致病性猪圆环病毒1型作为疫苗传递病毒载体表达猪繁殖与呼吸综合征病毒的抗原表位

A novel baculovirus vector shows efficient gene delivery of modified porcine reproductive and respiratory syndrome virus antigens and elicits specific immune response.

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