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A highly conserved epitope-vaccine candidate against varicella-zoster virus induces neutralizing antibodies in mice

机译：抗水痘带状疱疹病毒的高度保守的表位疫苗候选物诱导小鼠中和抗体

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摘要

Varicella-zoster virus (VZV) is a highly infectious agent of varicella and herpes zoster (HZ). Vaccination is by far the most effective way to prevent these diseases. More safe, stable and efficient vaccines, such as epitope-based vaccines, now have been increasingly investigated by many researchers. However, only a few VZV neutralizing epitopes have been identified to date. We have previously identified a linear epitope between amino acid residues 121 and 135 of gE. In this study, we validated that this epitope is highly conserved amongst different VZV strains that covered five existing phylogenetic clades with an identity of 100%. We evaluated the immunogenicity of the recombinant hepatitis B virus core (HBc) virus-like particles (VLPs) which included amino acids (121-135). VZV-gE-specific antibodies were detected in immunized mouse serum using ELISA. The anti-peptide antiserum positively detected VZV via Western blot and immunofluorescent staining assays. More importantly, these peptides could neutralize VZV, indicating that these peptides represented neutralizing epitopes. These findings have important implications for the development of epitope-based protective VZV vaccines. (C) 2016 Elsevier Ltd. All rights reserved.
机译：水痘带状疱疹病毒（VZV）是水痘和带状疱疹（HZ）的高度传染性因子。迄今为止，接种疫苗是预防这些疾病的最有效方法。更安全，稳定和高效的疫苗，例如基于表位的疫苗，现在已被许多研究人员日益研究。然而，迄今为止，仅鉴定了少数几个VZV中和表位。我们先前已经确定了gE的氨基酸残基121和135之间的线性表位。在这项研究中，我们验证了该表位在不同的VZV株之间是高度保守的，该株覆盖了五个现有的系统进化枝，其同一性为100％。我们评估了包含氨基酸（121-135）的重组乙型肝炎病毒核心（HBc）病毒样颗粒（VLP）的免疫原性。使用ELISA在免疫的小鼠血清中检测到VZV-gE特异性抗体。抗肽抗血清通过蛋白质印迹法和免疫荧光染色法阳性检测VZV。更重要的是，这些肽可以中和VZV，表明这些肽代表中和表位。这些发现对基于表位的保护性VZV疫苗的开发具有重要意义。 （C）2016 Elsevier Ltd.保留所有权利。

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