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首页> 外文期刊>Vaccine >IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets
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IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets

机译：通过水凝胶-碳纳米管复合物施用的抗肠毒素大肠杆菌的IgY可以预防实验性仔猪的新生儿腹泻

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摘要

In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulin is used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets. Groups of piglets orally challenged with 10(11) CFU/mL of ETEC were treated with non-protected and protected IgY. The clinical response of each group was monitored and evaluated in terms of dehydration, rectal temperature, faecal consistency score and body weight gain. Blood parameters and histological aspects were also studied. The results showed that treatment of infected piglets with protected IgY reduced significantly the severity of diarrhea. Non-protected IgY group show a lower recovery rate. Blood parameters and histological aspects were normal in both groups. Collectively, these results support previous in vitro studies showing that the nanocomposites can be an effective method of IgY protection against gastric inactivation. (C) 2016 Elsevier Ltd. All rights reserved.
机译：在以前的研究中，建立了聚合物水凝胶在保护蛋黄免疫球蛋白（IgY）免受模拟胃部疾病方面的适用性。此后，通过添加壳聚糖包裹的碳纳米管改善了水凝胶的性能，并评估了这些纳米复合材料对猪肠道细胞的体外毒性。本研究的目的是评估当免疫球蛋白用于攻击小猪的产肠毒素大肠杆菌（ETEC）时，纳米复合基质对IgY的保护作用。口服10（11）CFU / mL ETEC攻击的仔猪用未保护的和保护的IgY进行治疗。监测和评估每组的临床反应，包括脱水，直肠温度，粪便稠度评分和体重增加。还研究了血液参数和组织学方面。结果表明，用受保护的IgY处理受感染的仔猪可显着降低腹泻的严重程度。未保护的IgY组的恢复率较低。两组的血液参数和组织学方面均正常。总的来说，这些结果支持了先前的体外研究，表明纳米复合材料可以有效地保护IgY抵抗胃失活。 （C）2016 Elsevier Ltd.保留所有权利。

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