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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Acetate anion-triggered peroxygenation of non-native substrates by wild-type cytochrome P450s
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Acetate anion-triggered peroxygenation of non-native substrates by wild-type cytochrome P450s

机译：野生型细胞色素P450对非天然底物的乙酸根阴离子触发的过氧化

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摘要

Cytochrome P450(SP alpha) (P450(SP alpha)) and cytochrome P450(BS beta) (P450(BS beta)) belonging to the CYP152 family of enzymes (CYP152s) can utilize H2O2 efficiently as an oxidant for the generation of compound I. Although P450(SP alpha) and P450(BS beta) have very high substrate specificity and catalyse hydroxylation of long-chain fatty acids exclusively, we found that they can oxidize non-native substrates such as styrene simply by including medium chain length n-alkyl carboxylic acids as "decoy molecules." Although we had assumed that acetic acid did not serve as a decoy molecule, P450(SP alpha) and P450(BS beta) efficiently catalysed oxidation of non-native substrates when the reaction was carried out at a high concentration of acetate anion. The turnover rate for epoxidation of styrene catalysed by P450(BS beta) in the presence of 1 M acetate anion reached 590 (+/-) 30 min(-1).
机译：属于CYP152酶家族（CYP152s）的细胞色素P450（SP alpha）（P450（SP alpha））和细胞色素P450（BS beta）（P450（BS beta））可以有效地利用H2O2作为氧化剂来生成化合物I 。尽管P450（SP alpha）和P450（BS beta）具有很高的底物特异性，并专门催化长链脂肪酸的羟基化，但我们发现它们可以通过将中等链长的n-烷基羧酸作为“诱饵分子”。尽管我们假设乙酸不是诱饵分子，但是当在高浓度乙酸根阴离子下进行反应时，P450（SP alpha）和P450（BS beta）可以有效催化非天然底物的氧化。在1 M乙酸根阴离子存在下，P450（BS beta）催化的苯乙烯环氧化的周转速率达到590（+/-）30 min（-1）。

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