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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Reaction-based turn-on fluorescent probes with magnetic responses for Fe2+ detection in live cells
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Reaction-based turn-on fluorescent probes with magnetic responses for Fe2+ detection in live cells

机译：具有磁性反应的基于反应的开启式荧光探针，用于活细胞中的Fe2 +检测

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Iron is the most abundant nutritionally essential transition metal found in the human body. It plays important roles in various biological processes such as oxygen delivery, electron transport, enzymatic reactions and DNA synthesis and repair. However, iron can also catalyze the production of free radicals, which are linked to quite a few diseases such as cancer, neurodegenerative diseases, and cardiovascular diseases. Both iron deficiency and iron overload are related to various health problems. Thus, precisely monitoring iron ions (Fe2+ and Fe3+) in biological systems is important in understanding the detailed biological functions of iron and its trafficking pathways. However, effective tools for monitoring labile Fe2+ in biological systems have not yet been established. Reported herein are turn on, reaction-based coumarin and rhodamine-linked nitroxide probes (Cou-T and Rh-T) for selective detection of Fe2+ in solution and in living cells. Rh-T displayed a unique change in the EPR signal as well as enhancement of the fluorescence signal resulting from a specific redox reaction between the probe and Fe2+. The turn-on fluorescence response towards Fe2+ allows the subcellular imaging of endogenous Fe2+ as well as imaging under conditions of external iron supplementation or depletion, with a labile Fe2+ pool located in the mitochondria of human fibroblast primary cells. The detection and mechanism were verified by the magnetic properties of the probe via electron paramagnetic resonance (EPR) spectroscopy in solution and in cells.
机译：铁是人体中最丰富的营养必需的过渡金属。它在各种生物过程中发挥重要作用，例如氧气输送，电子传输，酶促反应以及DNA合成和修复。但是，铁还可以催化自由基的产生，这些自由基与很多疾病有关，例如癌症，神经退行性疾病和心血管疾病。铁缺乏和铁过载都与各种健康问题有关。因此，精确地监测生物系统中的铁离子（Fe2 +和Fe3 +）对于理解铁的详细生物学功能及其运输途径非常重要。但是，尚未建立用于监测生物系统中不稳定的Fe2 +的有效工具。本文报道了基于反应的香豆素和若丹明连接的一氧化氮探针（Cou-T和Rh-T），用于选择性检测溶液和活细胞中的Fe2 +。 Rh-T在EPR信号中表现出独特的变化，并且由于探针和Fe2 +之间的特定氧化还原反应而导致荧光信号增强。对Fe2 +的开启荧光反应允许内源性Fe2 +的亚细胞成像，以及在外部铁补充或消耗条件下的成像，其中不稳定的Fe2 +池位于人成纤维细胞原代细胞的线粒体中。通过溶液和细胞中的电子顺磁共振（EPR）光谱通过探针的磁性能验证了检测和机理。

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