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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Simultaneous Determination of Melamine and 5-Hydroxymethylfurfural in Milk by Capillary Electrophoresis with Diode Array Detection
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Simultaneous Determination of Melamine and 5-Hydroxymethylfurfural in Milk by Capillary Electrophoresis with Diode Array Detection

机译：毛细管电泳-二极管阵列检测法同时测定牛奶中的三聚氰胺和5-羟甲基糠醛

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摘要

This article describes the development of a simple analytical approach for the simultaneous determination of melamine and 5-hydroxymethylfurfural (HMF) in milk samples using capillary electrophoresis (CE) with diode array detection (DAD) for the first time. Ultraviolet absorption at wavelengths of 214 and 280 nm was applied for the detection of melamine and HMF, respectively. Milk samples were extracted with 1% trichloroacetic acid using a high-speed blender and ultrasonication. After centrifugation and filtration, the extract was analyzed by CE-DAD directly. Micellar electrokinetic capillary chromatography was employed as the separation mode by adding sodium dodecyl sulfate (SDS) to the electrolyte. Under optimal separation conditions, melamine, HMF, and interferents were well resolved. The linear dynamic ranges were 0.05-100 μg/mL for melamine (R~2 = 0.9996) and 0.1-100 μg/mL for HMF (H~2 = 0.9997). The assay detection limits were 0.047 μg/mL and 0.067, μg/mL for melamine and HMF, respectively. Satisfactory results were obtained for the assay recovery rate and repeatability. The proposed method was successfully applied for the analysis of melamine and HMF in real milk samples, and the results of melamine were comparable to those obtained using HPLC-UV reference method.
机译：本文介绍了使用毛细管电泳（CE）和二极管阵列检测（DAD）首次同时测定牛奶样品中三聚氰胺和5-羟甲基糠醛（HMF）的简单分析方法的发展。分别在214和280 nm的波长处进行紫外线吸收，以检测三聚氰胺和HMF。使用高速搅拌器和超声处理，用1％三氯乙酸提取牛奶样品。离心过滤后，提取物直接通过CE-DAD分析。通过向电解质中添加十二烷基硫酸钠（SDS），采用胶束电动毛细管色谱作为分离方式。在最佳分离条件下，三聚氰胺，HMF和干扰物得到了很好的分离。三聚氰胺的线性动态范围是0.05-100μg/ mL（R〜2 = 0.9996），HMF的线性动态范围是0.1-100μg/ mL（H〜2 = 0.9997）。三聚氰胺和HMF的检出限分别为0.047μg/ mL和0.067，μg/ mL。测定回收率和重复性均获得令人满意的结果。所提出的方法已成功地用于实际牛奶样品中三聚氰胺和HMF的分析，三聚氰胺的结果可与HPLC-UV参考方法获得的结果相媲美。

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