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首页> 外文期刊>Journal of Agricultural and Food Chemistry >A Fluoroimmunoassay Based on Quantum Dot-Streptavidin Conjugate for the Detection of Chlorpyrifos
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A Fluoroimmunoassay Based on Quantum Dot-Streptavidin Conjugate for the Detection of Chlorpyrifos

机译：基于量子点-链霉亲和素缀合物的荧光免疫分析法检测毒死rif

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摘要

A rapid and sensitive competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum dot-streptavidin conjugate (QDs-SA) was developed for the detection of chlorpyrifos in drinking water. The QDs-SA conjugate, which consists of 3-mercaptopropyl acid-stabilized CdTS nanoparticle QDs and streptavidin (SA) made through the active ester method, was employed to improve the sensitivity of QDs-SA-cFLISA. The 50% inhibition concentration (IC_(50)) and the limit of detection (LOD) were 28.5 and 3.8 ng mL~(-1), respectively. QDs-SA-cFLISA increased sensitivity 5.5-fold and reduced detection time by 1 h compared with conventional enzyme-linked immunosorbent assay (ELISA). With chlorpyrifos concentrations of 100, 50, and 20 ng mL~(-1), recoveries ranged from 85.9% to 105.3% with coefficients of variation ranging from 6.3% to 13.5%. This study demonstrated that QDs-SA-cFLISA Was more rapid and sensitive than conventional ELISA. Therefore, it can be used as a novel screening method for the detection of pesticide residues.
机译：建立了一种基于量子点-链霉亲和素偶联物（QDs-SA）的快速灵敏的竞争性荧光连接免疫吸附法（cFLISA），用于检测饮用水中的毒死rif。 QDs-SA共轭物由3-巯基丙酸稳定的CdTS纳米颗粒QDs和通过活性酯法制得的链霉亲和素（SA）组成，用于提高QDs-SA-cFLISA的敏感性。 50％抑制浓度（IC_（50））和检出限（LOD）分别为28.5和3.8 ng mL〜（-1）。与传统的酶联免疫吸附测定（ELISA）相比，QDs-SA-cFLISA的灵敏度提高了5.5倍，检测时间缩短了1小时。毒死rif的浓度分别为100、50和20 ng mL〜（-1）时，回收率为85.9％至105.3％，变异系数为6.3％至13.5％。这项研究表明，QDs-SA-cFLISA比常规ELISA更快，更灵敏。因此，它可以用作检测农药残留的新型筛选方法。

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