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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Determination of the Folate Content in Cladodes of Nopal (Opuntia ficus indica) by Microbiological Assay Utilizing Lactobacillus easel (ATCC 7469) and Enzyme-Linked Immunosorbent Assay
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Determination of the Folate Content in Cladodes of Nopal (Opuntia ficus indica) by Microbiological Assay Utilizing Lactobacillus easel (ATCC 7469) and Enzyme-Linked Immunosorbent Assay

机译：利用乳杆菌属画架（ATCC 7469）和酶联免疫吸附测定的微生物测定法测定胭脂（印度仙人掌）枝叶中的叶酸含量

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摘要

Prickly pear cactus has been an important food source in Mexico since ancient times due to its economical and ecological benefits and potential nutraceutical value. Nevertheless, studies on the nutritional aspects and health benefits have been scarce. The purpose of this study was to assess, apparently for the first time, the folate contents of cladodes of nopal by a microbiological assay, using Lactobacillus casei (ATCC 7469) in extracts that were enzymatically treated to release the bound vitamin, employing single, dual, and trienzymatic procedures, and using the enzyme-linked immunosorbent assay (ELISA). We used Opuntia cladodes of different length sizes. The microbiological assay showed some differences among enzyme treatments and sizes of nopal; the trienzyme treatment (α-amylase-protease-conjugase) was more efficient in determining the folate content in nopal, giving 5.0 ng/g in the small size cladodes at 54 h of testing time, while ELISA showed no significant differences in the folate content among sizes of cladodes (5.5-5.62 ng/g at 0 min testing time). Both techniques may be used for the assessment of folate content in cladodes, but ELISA is more rapid and reliable.
机译：自古以来，由于仙人掌仙人掌仙人掌的经济和生态效益以及潜在的保健价值，它一直是墨西哥的重要食物来源。然而，关于营养方面和健康益处的研究很少。这项研究的目的显然是第一次通过微生物学分析，使用干酪乳杆菌（ATCC 7469）对经酶处理以释放结合的维生素的提取物进行微生物学测定，采用单一，双重方法评估胭脂红的叶丛中的叶酸含量。 ，三酶法，并使用酶联免疫吸附测定（ELISA）。我们使用了不同长度尺寸的仙人掌包层。微生物测定显示酶处理和胭脂树的大小之间存在一些差异。三酶处理（α-淀粉酶-蛋白酶-缀合酶）更有效地测定胭脂红中的叶酸含量，在测试时间的54小时内，小包囊中的含量为5.0 ng / g，而ELISA显示叶酸含量没有显着差异在包层的尺寸之间（测试时间为0分钟时为5.5-5.62 ng / g）。两种技术都可用于评估枝叶中叶酸的含量，但ELISA更快速，更可靠。

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