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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Proteome Profiling of Seed Storage Proteins Reveals the Nutritional Potential of Salicomia brachiata Roxb., an Extreme Halophyte
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Proteome Profiling of Seed Storage Proteins Reveals the Nutritional Potential of Salicomia brachiata Roxb., an Extreme Halophyte

机译：种子贮藏蛋白的蛋白质组分析揭示了极端盐生植物Salicomia brachiata Roxb。的营养潜力。

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摘要

Salkornia brachiata is an extreme halophyte that grows in salty marshes and is considered to be a potential alternative crop for seawater agriculture. Salicomia seeds are rich in protein, and its tender shoots are eaten as salad greens. Seed storage proteins were fractionated by sequential extraction using different solvents, including distilled water for albumins, NaCl (1,0 M) for globulins, NaOH (0.1 N) for glutelins, and ethanol (70% v/v) for prolamine Globulins accounted for 54.75% of the total seed storage proteins followed by albumins (34.30%) and glutelins (8.70%). The fractionated proteins were characterized using 2D-diagonal SDS-PAGE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. The globulin fraction, composed of seven intermolecular disulfide-linked polypeptide pairs of molecular mass 63.5, 62.S, 54.7, 53.0, 43.2, 38.5, and 35.1 kDa, encompassed a basic and an acidic subunit. Two-dimensional gels revealed approximately 32 spots, with isoelectric points and molecular masses ranging from 4.93 to 11.6 and from ～5.2 to ～109.4 kDa, respectively. Protein spots were identified by MALDI-TOF MS peptide mass fingerprint analysis and further classified. Homology analysis demonstrated that 19% of the proteins were involved in metabolism, 16% were involved in signaling, and 15% were regulatory proteins. Peptide mass fingerprint analysis confirmed the presence of inter- and intramolecular disulfide linkages in the globulin fraction. Sulfur-rich proteins are of high nutritional value, and disulfides make S. brachiata a potential source of dietary supplementation.
机译：Salkornia brachiata是一种极端的盐生植物，生长在盐沼中，被认为是海水农业的潜在替代作物。 ali肉种子富含蛋白质，嫩嫩的嫩芽可作为蔬菜沙拉食用。通过使用不同溶剂的顺序萃取来分离种子贮藏蛋白，其中蒸馏水用于白蛋白，NaCl（1,0 M）用于球蛋白，NaOH（0.1 N）用于谷蛋白，而乙醇（70％v / v）用于谷蛋白谷蛋白种子储存蛋白总量的54.75％，其次是白蛋白（34.30％）和谷蛋白（8.70％）。使用2D对角SDS-PAGE和基质辅助的激光解吸/电离飞行时间（MALDI-TOF）质谱对分离的蛋白质进行表征。球蛋白部分由七个分子质量为63.5、62.S，54.7、53.0、43.2、38.5和35.1 kDa的分子间二硫键连接的多肽组成，涵盖一个碱性和一个酸性亚基。二维凝胶显示大约32个斑点，等电点和分子量分别为4.93至11.6和〜5.2至〜109.4 kDa。蛋白斑点通过MALDI-TOF MS肽质量指纹分析鉴定并进一步分类。同源性分析表明，19％的蛋白质参与代谢，16％的蛋白质参与信号传导，15％的蛋白质是调节蛋白。肽质量指纹分析证实了球蛋白级分中存在分子间和分子内二硫键。富含硫的蛋白质具有很高的营养价值，而二硫化物使腕带链球菌成为膳食补充的潜在来源。

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