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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Quantification of Flavonoids in Black Rice by Liquid Chromatography-Negative Electrospray Ionization Tandem Mass Spectrometry
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Quantification of Flavonoids in Black Rice by Liquid Chromatography-Negative Electrospray Ionization Tandem Mass Spectrometry

机译:液相色谱-负电喷雾电离串联质谱法定量分析黑米中的类黄酮

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摘要

Systematic identification and structural characterization of flavonoids and their glycosides in bran extracts of seven Thai black rice varieties were performed by sequential uses of reversed-phase HPLC with a photodiode array detector and a combined electrospray ionization tandem mass spectrometer. Eleven flavonoids were detected, and six of these were found for the first time in rice bran. These were taxifolin-7-O-glucoside, myricetin-7-O-glucoside, isorhamnetin-3-O-acetylglucoside, isorhamnetin-7-O-rutinoside, 5,6,3',4',5'-pentahydroxyflavone-7-O-glucoside, and 5,3',4',5'-tetrahydroxyflavanone-7-O-glucoside. The quantitative results revealed that different rice varieties possessed flavonoids in different concentrations. The most abundant glycoside derivative of flavonoids widely distributed among the rice varieties was monoglucoside, such as quercetin-3-O-glucoside, isorhamnetin-3-O-glucoside, and isorhamnetin-3-O-glucoside.
机译:通过连续使用反相HPLC和光电二极管阵列检测器和组合电喷雾串联质谱仪,对七个泰国黑米品种的麸皮提取物中的黄酮类化合物及其糖苷进行系统鉴定和结构表征。共检测到11种黄酮类化合物,其中有6种是首次在米糠中发现。这些是滑石粉-7-O-葡糖苷,杨梅素-7-O-葡糖苷,异鼠李素-3-O-乙酰葡糖苷,异鼠李素-7-O-芸香糖苷,5,6,3',4',5'-五羟基黄酮-7 -O-葡糖苷和5,3',4',5'-四羟基黄烷酮-7-O-葡糖苷。定量结果表明,不同水稻品种具有不同浓度的黄酮类化合物。在水稻品种中分布最丰富的类黄酮最丰富的糖苷衍生物是单糖苷,例如槲皮素-3-O-葡萄糖苷,异鼠李素-3-O-葡萄糖苷和异鼠李素-3-O-葡萄糖苷。

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