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首页> 外文期刊>Journal of Agricultural and Food Chemistry >A Murine Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for Almond (Prunus dulcis L) Detection
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A Murine Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for Almond (Prunus dulcis L) Detection

机译:一种基于鼠单克隆抗体的酶联免疫吸附法检测杏仁(李)

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摘要

A sandwich enzyme-linked immunosorbent assay (ELISA) using anti-almond soluble protein rabbit polyclonal antibodies as capture antibodies and murine monoclonal antibody 4C10 as the detection antibodies was developed. The assay is specific and sensitive (3—200 ng almond protein/mL) for almond detection. The standardized assay is accurate (<15% CV) and reproducible (intra- and inter assay variability <15% CV). The assay did not register any cross-reactivity with the tested food matrices, suggesting the assay to be almond amandin specific. The assay could detect the presence of declared almond in the tested matched commercial samples. Further, the assay reliably detected the presence of almonds in the laboratory prepared food samples spiked with almond flour.
机译:建立了以抗杏仁可溶性蛋白兔多克隆抗体为捕获抗体,鼠单克隆抗体4C10作为检测抗体的三明治酶联免疫吸附试验(ELISA)。该检测方法对杏仁检测具有特异性和敏感性(3-200 ng杏仁蛋白/ mL)。标准化测定准确(<15%CV)且可重复(测定内和测定间变异性<15%CV)。该测定法与被测食品没有任何交叉反应,表明该测定法是杏仁金刚烷特异性的。该测定法可以检测测试的匹配商业样品中是否存在申报的杏仁。此外,该测定法可靠地检测了掺有杏仁粉的实验室制备食物样品中杏仁的存在。

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