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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Hyperspectral Deep Ultraviolet Autofluorescence of Muscle Fibers Is Affected by Postmortem Changes
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Hyperspectral Deep Ultraviolet Autofluorescence of Muscle Fibers Is Affected by Postmortem Changes

机译：验尸变化影响肌肉纤维的高光谱深紫外线自发荧光

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After slaughter, muscle cells undergo biochemical and physicochemical changes that may affect their autofluorescence characteristics. The autofluorescent response of different rat extensor digitorum lougus (EDL) and soleus muscle fiber types was investigated by deep ultraviolet (UV) synchrotron microspectroscopy immediately after animal sacrifice and after 24 h of storage in a moist chamber at 20 degrees C. The glycogen content decreased from 23 to 18 mu mol/g of fresh muscle in 24 h postmortem. Following a 275 urn excitation wavelength, the spectral muscle fiber autofluotescence response showed discrimination depending upon postmortem time (t(0) versus t(24) h) on both muscles at 346 and 302 nm and, to a lesser extent, at 408 and 325 nm. Taken individually, all fiber types were discriminated but with variable accuracy, with type IIA. showing better separation of t(0)/t(24) h than other fiber types. These results suggest the usefulness of the autofluorescent response of muscle cells for rapid meat-aging characterization.
机译：宰杀后，肌肉细胞发生生化和物理化学变化，可能会影响其自身荧光特性。在处死动物后以及在20°C的潮湿室内保存24小时后，立即通过深紫外（UV）同步加速器显微术研究了不同大鼠大指趾长肌（EDL）和比目鱼肌纤维类型的自发荧光反应。死后24小时内从23到18摩尔/克的新鲜肌肉。在275 n激发波长之后，频谱肌纤维自发荧光反应显示了在346和302 nm处以及在较小程度上在408和325处，取决于死后时间（t（0）对t（24）h）的区分。纳米单独考虑，使用IIA型可以区分所有光纤类型，但精度不同。显示出比其他纤维类型更好的t（0）/ t（24）h分离度。这些结果表明，肌肉细胞的自发荧光反应对于快速老化肉的表征是有用的。

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