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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale
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Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale

机译：铁皮石Poly多糖的结构表征及免疫调节作用

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摘要

A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker, of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and H-1 and C-13 NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4 -linked beta-D-mannopyranosyl and beta-D-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-alpha secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.
机译：已确定粗制多糖成分（cDOP）是亚洲昂贵茶石D石D的特征标记，但尚未对其化学和生物活性进行研究。在此处报道的工作中，将cDOP脱淀粉（DOP，产率90％），并分为两个亚级多糖DOPa和DOPb，其特征在于单糖组成，甲基化分析和光谱分析（FT-IR和H-1和C-13） NMR）。两者都由甘露糖和葡萄糖以相似的比例组成，并且具有相似的结构，具有1,4-连接的β-D-甘露吡喃糖基和β-D-吡喃葡萄糖基残基的主链。仅在分子量上观察到显着差异。使用小鼠巨噬细胞系RAW264.7进行的生物测定表明，DOP及其两个亚组分以剂量依赖的方式增强细胞增殖，TNF-α分泌和吞噬作用。他们还单独和与有丝分裂原一起诱导淋巴细胞的增殖。因此，DOPa和DOPb被证明是D. officinale的主要活性多糖标记。

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