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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Structure Elucidation, Relative LC-MS Response and In Vitro Toxicity of Azaspiracids 7-10 Isolated from Mussels (Mytilus edulis)
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Structure Elucidation, Relative LC-MS Response and In Vitro Toxicity of Azaspiracids 7-10 Isolated from Mussels (Mytilus edulis)

机译：贻贝（Mytilus edulis）分离的氮杂螺菌酸7-10的结构阐明，相对LC-MS反应和体外毒性

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摘要

Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7-10, 7-10, were isolated from shellfish and their structures, previously proposed on the basis of only LC-MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4-6, 4-6, and 7-10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC-MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a time- and concentration-dependent manner; however, there were distinct differences in their EC50 values, with the potencies for each analogue being: AZA6 > AZA8 > AZA1 > AZA4 approximate to AZA9 > AZA5 approximate to AZA10. This data contributes to the understanding of the structure-activity relationships of AZAs.
机译：氮杂螺菌酸（AZA）是由鞭毛藻产生的海洋生物毒素，可在贝类中蓄积，如果食用，会导致中毒事件。从贝类中分离出AZA7-10、7-10，以前仅根据LC-MS / MS数据提出的结构已通过NMR光谱法确认。纯化的AZA4-6、4-6和7-10通过qNMR准确定量，并首次用于检测Jurkat T淋巴细胞的细胞毒性。使用等度洗脱和梯度洗脱在选定的离子监测和选定的反应监测模式下进行的LC-MS（MS）摩尔响应研究表明，相对于AZA1，1，类似物的响应范围为0.3至1.2。 T淋巴细胞具有时间和浓度依赖性;但是，它们的EC50值存在明显差异，每种类似物的效价分别为：AZA6> AZA8> AZA1> AZA4近似于AZA9> AZA5近似于AZA10。该数据有助于理解AZA的结构-活性关系。

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