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首页> 外文期刊>Journal of Applied Polymer Science >Immobilization of lipase on a polymeric microsphere with an epoxy group prepared by radiation-induced polymerization
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Immobilization of lipase on a polymeric microsphere with an epoxy group prepared by radiation-induced polymerization

机译：通过辐射诱导的聚合反应制备具有环氧基的脂肪酶固定在聚合物微球上

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摘要

A polymeric microsphere (PM) with an epoxy group was prepared by the radiation-induced polymerization of glycidyl methacrylate and diethylene glycol dimethacrylate in reaction conditions with variations in solvents, irradiation dose, and monomer composition. The epoxy group of the PM was analyzed by solid-state C-13-NMR, Fourier transform infrared spectroscopy (FTIR), Fourier transform Raman spectroscopy, and elemental analysis (EA) after amination. In EA after amination, the epoxy group content was in the range 0.20-0.50 mmol/g. The lipase was immobilized to the epoxy group of the PM in experimental conditions with variations in the pH and the epoxy group content. The activity of the lipase-immobilized PM was in the range 148-342 unit/mg min. The activity of lipase-immobilized PM increased in accordance with the epoxy group content. The lipase-immobilized PM was also characterized by FTIR and EA. (C) 2003 Wiley Periodicals, Inc. [References: 15]
机译：通过在溶剂，辐照剂量和单体组成变化的反应条件下，通过辐射诱导的甲基丙烯酸缩水甘油酯和二甘醇二甲基丙烯酸甲酯的辐射诱导聚合反应，制备了具有环氧基的聚合物微球（PM）。胺化后，通过固态C-13-NMR，傅里叶变换红外光谱（FTIR），傅里叶变换拉曼光谱和元素分析（EA）分析PM的环氧基。在胺化后的EA中，环氧基含量在0.20-0.50mmol / g的范围内。在pH和环氧基含量变化的实验条件下，将脂肪酶固定在PM的环氧基上。固定有脂肪酶的PM的活性为148-342单位/ mg min。固定有脂肪酶的PM的活性随环氧基含量的增加而增加。固定有脂肪酶的PM的特征还在于FTIR和EA。 （C）2003 Wiley Periodicals，Inc. [参考：15]

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