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首页> 外文期刊>Journal of Applied Polymer Science >Development of Biosensor for Phenol Detection Using Agarose-Guar Gum Based Laccases Extracted from Pleurotus ostreatus
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Development of Biosensor for Phenol Detection Using Agarose-Guar Gum Based Laccases Extracted from Pleurotus ostreatus

机译：平菇提取的基于琼脂糖-瓜尔胶的漆酶检测苯酚的生物传感器的研制

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摘要

Laccases from Pleurotus ostreatus was extracted from the Shaken flask cultures of Pleurotus ostreatus grown at 25 degrees C with continuous agitation (110 rpm.) in baffled 1000 mL Erlenmeyer flasks containing 200 mL medium. The basal GYP medium used for cultures contained 20 g glucose l(-1), 5 g yeast extract l(-1), 5 g peptone from casein l(-1), and 1 g MgSO4.7H(2)O l(-1). The pH was adjusted to 5.0 with H3PO4 before sterilization. The kinetics of oxidation reactions catalyzed by laccases was studied using 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid). The laccases showed lower specific activity and higher activity in nonpolar organic solvents. A biosensor using laccases was constructed for the determination of phenol. The enzyme was extracted from Pleurotus ostreatus and entrapped in agarose-guar gum composite biopolymer matrix. Phenol was determined by direct reduction of biocatalytically liberated quinone species at -0.1 V versus Ag/AgCl (3M KCl). The response was found to be linear and concentration dependent. It has a shelf life of more than 2 months when stored at 4 degrees C.
机译：从平菇的摇瓶培养物中提取平菇的漆酶，该摇瓶培养物在装有200 mL培养基的1000 mL锥形烧瓶中，在连续搅拌（110 rpm）下于25摄氏度下生长。用于培养的基础GYP培养基包含20 g葡萄糖l（-1），5 g酵母提取物l（-1），5 g酪蛋白l（-1）的蛋白ept和1 g MgSO4.7H（2）O l（ -1）。灭菌前，用H3PO4将pH调至5.0。使用2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）研究了漆酶催化的氧化反应动力学。漆酶在非极性有机溶剂中显示出较低的比活性和较高的活性。构造了使用漆酶的生物传感器，用于测定苯酚。从平菇侧耳提取酶，并包埋在琼脂糖-瓜尔胶合成生物聚合物基质中。苯酚是通过在相对于Ag / AgCl（3M KCl）的-0.1 V下直接还原生物催化释放的醌种类而确定的。发现该反应是线性的并且是浓度依赖性的。在4摄氏度下保存时，其保质期超过2个月。

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