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首页> 外文期刊>Journal of Applied Polymer Science >Effect of polyethylene glycol tether size and chemistry on the attachment of lactase to polyethylene films
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Effect of polyethylene glycol tether size and chemistry on the attachment of lactase to polyethylene films

机译：聚乙二醇束带的大小和化学性质对乳糖酶在聚乙烯膜上附着的影响

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摘要

Numerous foodstuffs have been developed for lactose intolerant individuals. However, current methods to reduce lactose require excessive enzyme and are not viable for small producers. A novel solution proposed to overcome these problems is the immobilization of lactase to standard food-contact packaging materials. The ability of these packages to convert lactose is dependent on the quantity and specific activity of the enzyme after immobilization. In this study, the material interface of polyethylene (PE) films was modified with polyethylene glycol (PEG) tethers of 1 kDa, 2 kDa, 5 kDa, and 10 kDa containing epoxy, acrylate, aldehyde, and succinimdyl ester end-group functionality. The results showed that a 5 kDa tether enabled maximum absolute activity of 0.49 × 10 ~(-2) ALU/cm ~2 when compared to tethers of different sizes with identical functionalization. Compared to succinimdyl, epoxide, and acrylate functionalized PEG tethers, an aldehyde end-group resulted in films with the highest absolute immobilized enzyme activity of 0.67 × 10 ~(-2) ALU/cm ~2. Regardless of tether size or chemistry, the activity retention of lactase was less than 15% compared to the soluble enzyme after immobilization to PEG-functionalized PE films.
机译：已经为乳糖不耐症的人开发了许多食品。然而，当前减少乳糖的方法需要过量的酶，并且对于小规模生产者是不可行的。为克服这些问题而提出的新颖解决方案是将乳糖酶固定在与食品接触的标准包装材料上。这些包装物转化乳糖的能力取决于固定后酶的数量和比活性。在这项研究中，聚乙烯（PE）薄膜的材料界面用1 kDa，2 kDa，5 kDa和10 kDa的聚乙二醇（PEG）系链进行了修饰，其中包含环氧，丙烯酸酯，醛和琥珀酰亚胺酯端基官能团。结果表明，与功能相同的不同大小的系链相比，5 kDa系链的最大绝对活性为0.49×10〜（-2）ALU / cm〜2。与琥珀酰亚胺基，环氧化物和丙烯酸酯官能化的PEG系链相比，醛端基产生的膜的绝对固定化酶活性最高，为0.67×10〜（-2）ALU / cm〜2。无论系链大小或化学性质如何，固定在PEG-官能化PE膜上后，乳糖酶的活性保留率均低于可溶性酶的15％。

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