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首页> 外文期刊>Journal of Applied Polymer Science >In situ gelling polysaccharide-based hydrogel for cell and drug delivery in tissue engineering
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In situ gelling polysaccharide-based hydrogel for cell and drug delivery in tissue engineering

机译：用于组织工程中细胞和药物输送的原位凝胶化多糖基水凝胶

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摘要

Injectable hydrogels have attracted a great deal of attention as cell carriers and bioactive agents in regenerative medicine due to their ability to fill complex three-dimensional (3D) tissue gaps and relative ease of in vivo administration. Polysaccharide-based hydrogels can provide microenvironments that favor tissue regeneration and biocompatibility due to their chemical similarities with native extracellular matrix components. This manuscript reports the in vitro application of an injectable chitosan-based polysaccharide hydrogel for cell and protein delivery. Crosslinked hydrogels were produced by the reaction between the amino functionality of chitosan and the aldehyde of dextran aldehyde resulting in an imine bond (Schiff's base) formation in aqueous solutions. This approach eliminated the use of additional crosslinking agents which may pose undesired side effects regarding cytotoxicity and biocompatibility. Additionally, we demonstrate versatility of the gel in terms of its fabrication, and ability to alter mechanical properties by changing the crosslinking extent due to aldehyde content. Bovine serum albumin (BSA), used as a model protein, followed a steady release pattern from the gel. BSA release was dependent on the extent of hydrogel crosslinking. Increase in crosslinking extent resulted in improved mechanical properties and sustained release of BSA. Human fetal osteoblasts encapsulated into the hydrogel showed at least 70% viability and continued to proliferate under in vitro culture.
机译：可注射水凝胶作为细胞载体和生物活性剂在再生医学中引起了广泛的关注，这是由于它们具有填补复杂的三维（3D）组织间隙的能力以及体内给药的相对简便性。基于多糖的水凝胶由于其与天然细胞外基质成分的化学相似性，可提供有利于组织再生和生物相容性的微环境。该手稿报道了可注射的壳聚糖多糖水凝胶在细胞和蛋白质输送中的体外应用。壳聚糖的氨基官能团与葡聚糖醛的醛之间的反应产生了交联的水凝胶，在水溶液中形成了亚胺键（席夫碱）。该方法消除了使用额外的交联剂，这可能对细胞毒性和生物相容性造成不良的副作用。此外，我们证明了凝胶的通用性，包括其制造能力以及通过改变醛含量引起的交联程度来改变机械性能的能力。用作模型蛋白的牛血清白蛋白（BSA）遵循从凝胶中稳定释放的模式。 BSA的释放取决于水凝胶交联的程度。交联程度的增加导致改善的机械性能和BSA的持续释放。封装在水凝胶中的人类胎儿成骨细胞显示出至少70％的活力，并在体外培养下继续增殖。

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