• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >Vinculin focal adhesion of osteoblast-like cells on PEEK coated with ultra-thin polymer nano films
【24h】

Vinculin focal adhesion of osteoblast-like cells on PEEK coated with ultra-thin polymer nano films

机译：超薄聚合物纳米膜包被的PEEK上成骨细胞样细胞的Vinculin黏附

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PEEK is the polymer of choice to replace metal encapsulants and other parts in active medical implants fixated into bone. The current challenge is to improve its biocompatibility with bone tissue to ultimately achieve osseointegration. PEEK sheets surfaces coated with plasma deposited nano thin polymer films using CH4, (CH4+O-2) and (CH4+N-2) gases. PEEK samples plasma treated with nonpolymerizing gases (O-2) were also used for comparison. The adhesion performance of osteoblast like cells on the plasma-treated PEEK surfaces and the presence of Vinculin in these cells were evaluated after long culturing period (12 days). X-ray photoelectron spectroscopy and Auger spectroscopy were used to provide surface molecular information, surface hardness and molecular density. All plasma-treated surfaces retained functionality after the sterilization process. PEEK surfaces with high number of oxygen functional groups and particularly oxygen rich thin polymer coating (plasma deposition using CH4+O-2 gas mixture) resulted in strong cellular adhesion strength and large Vinculin amount. Further, osteoblast-like cells responded better to surfaces with lower molecular density acting like another signal for cell adhesion. The osteoblast-like cells response was weaker for surfaces with both thin films with nitrogen functional groups and nonfunctional (nonpolar) films. Furthermore, thin films rich in nitrogen functional groups repelled the cells, showed abnormal cells shape, smaller Vinculin amount and induced thicker cellular clusters with poor spread. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42181.
机译：PEEK是替代固定在骨骼中的有源医疗植入物中的金属密封剂和其他零件的首选聚合物。当前的挑战是改善其与骨组织的生物相容性，以最终实现骨整合。 PEEK板表面使用CH4，（CH4 + O-2）和（CH4 + N-2）气体等离子沉积的纳米聚合物薄膜涂覆。用非聚合气体（O-2）等离子处理过的PEEK样品也用于比较。经过长时间的培养（12天）后，评估了成骨细胞样细胞在经等离子体处理的PEEK表面上的粘附性能以及Vinculin在这些细胞中的存在。 X射线光电子能谱和俄歇能谱用于提供表面分子信息，表面硬度和分子密度。在灭菌过程之后，所有经过等离子体处理的表面都保留了功能。具有大量氧官能团的PEEK表面，尤其是富氧的薄聚合物涂层（使用CH4 + O-2气体混合物进行的等离子体沉积）导致强大的细胞粘附强度和大量的Vinculin。此外，成骨样细胞对较低分子密度的表面反应更好，就像另一个细胞粘附信号一样。具有氮官能团的薄膜和无功能（非极性）薄膜的表面的成骨样细胞反应均较弱。此外，富含氮官能团的薄膜会排斥细胞，显示出异常的细胞形状，较小的Vinculin量并诱导较厚的细胞簇，且扩散较差。 （c）2015 Wiley Periodicals，Inc. J. Appl。 Polym。科学2015，132，42181。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号