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首页> 外文期刊>Journal of Applied Polymer Science >New method for fabricating an alpha-fetoprotein affinity monolithic polymer array
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New method for fabricating an alpha-fetoprotein affinity monolithic polymer array

机译：制造甲胎蛋白亲和性整体聚合物阵列的新方法

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Bisphenol A based epoxy acrylate (BABEA), a commercial UV-curable material, was introduced as a crosslinker for the fabrication of an epoxy-functionalized monolithic polymer array through UV-initiated copolymerization with glycidyl methacrylate as the functional monomer and poly(ethylene glycol) 200 as the porogen. Scanning electron microscopy images showed that the monolithic poly(bisphenol A based epoxy acrylate-co-glycidyl methacrylate) [poly(BABEA-co-GMA)] exhibited a well-controlled skeletal and well-distributed porous structure. The alpha-fetoprotein (AFP) immunoaffinity monolithic polymer array prepared by the immobilization of AFP on epoxy-functionalized monolithic arrays was used as an immunosensor for chemiluminescent AFP detection. X-ray photoelectron spectroscopy results indicate that the AFP antibody was successfully immobilized on the monolithic poly(BABEA-co-GMA) array. With a noncompetitive immune-response format, the proposed AFP immunoaffinity array was demonstrated as a lowcost, flexible, homogeneous, and stable array for AFP detection. (C) 2014 Wiley Periodicals, Inc.
机译：引入双酚A基环氧丙烯酸酯（BABEA），一种可商购的UV固化材料，作为交联剂，通过与甲基丙烯酸缩水甘油酯作为功能单体和聚乙二醇的UV引发的共聚反应，制造环氧官能化的整体聚合物阵列200作为致孔剂。扫描电子显微镜图像显示，整体式聚（双酚A基环氧丙烯酸酯-甲基丙烯酸缩水甘油酯）[聚（BABEA-co-GMA）]表现出良好控制的骨架和良好分布的多孔结构。通过将AFP固定在环氧官能化的整体阵列上制备的甲胎蛋白（AFP）免疫亲和整体式聚合物阵列用作化学发光AFP检测的免疫传感器。 X射线光电子能谱结果表明，AFP抗体已成功固定在整体式聚（BABEA-co-GMA）阵列上。使用非竞争性免疫反应形式，提出的AFP免疫亲和力阵列被证明是一种低成本，灵活，均质且稳定的AFP检测阵列。 （C）2014威利期刊公司

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