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首页> 外文期刊>Journal of Applied Polymer Science >Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization
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Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization

机译：两步分散聚合法高效吸附蛋白质的磁性聚（苯乙烯-甲基丙烯酸缩水甘油酯）微球的制备与表征

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摘要

Micrometer-sized superparamagnetic poly(styrene-glycidyl methacrylate)/Fe3O4 spheres were synthesized by two-stage dispersion polymerization with modified hydrophobic Fe3O4 nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe3O4-P (St-GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 mu m in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe3O4-P (St-GMA) microspheres with immobilized iminodiacetic acid-Cu2+ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high-performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. (C) 2015 Wiley Periodicals, Inc.
机译：通过两步分散聚合，用改性的疏水性Fe3O4纳米粒子，苯乙烯（St）和甲基丙烯酸缩水甘油酯（GMA）合成微米级的超顺磁性聚（甲基丙烯酸苯乙烯-甲基丙烯酸缩水甘油酯）/ Fe3O4球。磁性Fe3O4-P（St-GMA）微球的形貌和性质通过扫描电子显微镜，透射电子显微镜，振动样品磁强法，热重分析和衰减的全反射率进行了检查。所得到的磁性微球的平均粒径为1.50μm，粒径分布窄，磁性微球的饱和磁化强度为8.23emu / g。固定有亚氨基二乙酸-Cu2 +基团的磁性Fe3O4-P（St-GMA）微球用于研究模型蛋白，牛血红蛋白（BHb）和牛血清白蛋白（BSA）的吸附能力和选择性。我们发现，通过高效液相色谱法测定，BHb的吸附能力高达190.66 mg / g的微球，是BSA吸附能力的59.64 mg / g的3.20倍。这些微球具有相当低的非特异性吸附，在蛋白质分离和纯化应用中具有巨大潜力。 （C）2015年Wiley Periodicals，Inc.

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