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首页> 外文期刊>Journal of Applied Polymer Science >Performance assessment of protein electrophoresis by using polyacrylamide hydrogel with porous structure modified with SDS micelles as template
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Performance assessment of protein electrophoresis by using polyacrylamide hydrogel with porous structure modified with SDS micelles as template

机译：以SDS胶束修饰的多孔结构聚丙烯酰胺水凝胶为模板的蛋白质电泳性能评估

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摘要

Separation processes are routinely used worldwide in biotechnology, chemical processing, wastewater treatment, and myriad other areas. Gel electrophoresis has been used for decades as a standard technique to separate charged biopolymers, such as DNA, RNA, and proteins. In this research, polyacrylamide hydrogels were synthesized in the presence of sodium dodecyl sulfate (SDS) micelles as nanotemplating agents in an effort to modify the internal porous microstructure of the gels. After removing these agents via a combination of passive and facilitated means, the gels were used during electrophoresis in order to assess the effects of its modified porous microstructure on protein separations. The results revealed that hydrogels containing 9% acrylamide and templated with SDS micelles in a 5-15% concentration range were the most effective materials in separating proteins in a range of 10-250 kDa. As expected, standard, nontemplated gels also resulted in separation of the proteins but not to the same extent as with the templated hydrogels. In summary, this research highlights the important role of the templating agent as possible useful tuning factors for achieving electrophoresis-based separation. (C) 2016 Wiley Periodicals, Inc.
机译：在世界范围内，分离工艺在生物技术，化学加工，废水处理以及其他众多领域中都被常规使用。凝胶电泳作为分离带电生物聚合物（例如DNA，RNA和蛋白质）的标准技术已使用了数十年。在这项研究中，聚丙烯酰胺水凝胶是在十二烷基硫酸钠（SDS）胶束作为纳米模板剂的存在下合成的，旨在改变凝胶的内部多孔微结构。通过被动和便利手段相结合去除这些试剂后，在电泳过程中使用凝胶，以评估其修饰的多孔微结构对蛋白质分离的影响。结果表明，含有9％丙烯酰胺并以5-15％浓度范围的SDS胶束为模板的水凝胶是分离10-250 kDa范围内蛋白质的最有效材料。不出所料，标准的非模板化凝胶也导致蛋白质分离，但程度不如模板化水凝胶。总而言之，这项研究强调了模板剂的重要作用，它是实现基于电泳的分离的可能有用的调节因子。 （C）2016威利期刊公司

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