• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cell Science >Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
【24h】

Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length

机译：Septins 2、7、9和MAP4沿着轴突在原发纤毛中共定位并控制纤毛长度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9 and that the three members of this complex colocalize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.
机译：Septins是一个庞大的，进化上保守的GTPases家族，它们形成异源寡聚体并与基于肌动蛋白的细胞骨架和微管相互作用。它们参与脚手架功能，并在发芽的酵母，精子鞭毛和肾上皮细胞的初级纤毛的基底中形成扩散屏障。我们研究了Septins在视网膜色素上皮（RPE）细胞的初级纤毛中的作用，发现SEPT2与SEPT7和SEPT9形成1：1：1的复合物，并且该复合物的三个成员沿轴突的长度共定位。与在肾上皮细胞中观察到的相似，通过基于siRNA的方法消除了纤毛定位的Septins抑制了纤毛发生。 MAP4是SEPT2的结合伴侣，它控制着Septins进入微管的通路，也被定位于轴突酶，在轴突看来对纤毛的长度有负调节作用。综上所述，我们的数据为细胞中主要纤毛和微管活动的组织中Septins和MAP4的功能和调控提供了新的见解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号