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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Off-line comprehensive 2-dimensional hydrophilic interaction x reversed phase liquid chromatography analysis of procyanidins
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Off-line comprehensive 2-dimensional hydrophilic interaction x reversed phase liquid chromatography analysis of procyanidins

机译：离线全面二维亲水相互作用x原花青素的反相液相色谱分析

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摘要

The development of an off-line comprehensive 2-dimensional liquid chromatography (2-D-LC) method for the analysis of procyanidins is reported. In the first dimension, oligomeric procyanidins were separated according to molecular weight by hydrophilic interaction chromatography (HILIC), while reversed phase LC was employed in the second dimension to separate oligomers based on hydrophobicity. Fluorescence, UV and electrospray ionisation mass spectrometry (ESI-MS) were employed for identification purposes. The combination of these orthogonal separation methods is shown to represent a significant improvement compared to 1-dimensional methods for the analysis of complex high molecular weight procyanidin fractions, by simultaneously providing isomeric and molecular weight information. The low correlation (r(2) < 0.2100) between the two LC modes afforded a practical peak capacity in excess of 2300 for the optimal off-line method. The applicability of the method is demonstrated for the analysis of phenolic extracts of apple and cocoa.
机译：报道了用于分析原花青素的离线全面二维液相色谱（2-D-LC）方法的开发。在第一维中，通过亲水相互作用色谱法（HILIC）根据分子量分离低聚原花青素，而在第二维中，采用反相LC根据疏水性分离低聚物。荧光，紫外和电喷雾电离质谱（ESI-MS）用于鉴定目的。与同时分析异构体和分子量信息的一维方法相比，这些正交分离方法的组合与一维分析复杂高分子量原花青素组分的分析相比，具有明显的改进。两种LC模式之间的低相关性（r（2）<0.2100）为最佳离线方法提供了超过2300的实用峰值容量。证明了该方法在分析苹果和可可酚提取物中的适用性。

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