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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Chromatographic biopanning for the selection of peptides with high specificity to Pb~(2+) from phage displayed peptide library
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Chromatographic biopanning for the selection of peptides with high specificity to Pb~(2+) from phage displayed peptide library

机译：色谱生物淘选技术从噬菌体展示的肽库中选择对Pb〜（2+）具有高特异性的肽

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摘要

Toxic heavy metal pollution is a global problem occurring in air, soil as well as water. There is a need for a more cost effective, renewable remediation technique, but most importantly, for a recovery method that is selective for one specific metal of concern. Phage display technology has been used as a powerful tool in the discovery of peptides capable of exhibiting specific affinity to various metals or metal ions. However, traditional phage display is mainly conducted in batch mode, resulting in only one equilibrium state hence low-efficiency selection. It is also unable to monitor the selection process in real time mode. In this study, phage display technique was incorporated with chromatography procedure with the use of a monolithic column, facilitating multiple phage-binding equilibrium states and online monitoring of the selection process in search of affinity peptides to Pb~(2+). In total, 17 candidate peptides were found and their specificity toward Pb~(2+) was further investigated with bead-based enzyme immunoassay (EIA). A highly specific Pb~(2+) binding peptide ThrAsnThrLeuSerAsnAsn (TNTLSNN) was obtained. Based on our knowledge, this is the first report on a new chromatographic biopanning method coupled with monolithic column for the selection of metal ion specific binding peptides. It is expected that this monolith-based chromatographic biopanning will provide a promising approach for a high throughput screening of affinity peptides cognitive of a wide range of target species.
机译：有毒重金属污染是空气，土壤和水中普遍存在的问题。需要一种更具成本效益的，可再生的修复技术，但最重要的是，需要一种对一种特定金属具有选择性的回收方法。噬菌体展示技术已被用作发现能够对各种金属或金属离子表现出特定亲和力的肽的有力工具。然而，传统的噬菌体展示主要以批处理模式进行，导致仅一种平衡状态，因此选择效率低。它也无法实时监控选择过程。在这项研究中，将噬菌体展示技术与色谱程序结合在一起，使用整体柱，以促进多种噬菌体结合平衡状态并在线监测选择过程，以寻找对Pb〜（2+）的亲和肽。总共发现了17种候选肽，并通过基于珠的酶免疫分析（EIA）进一步研究了其对Pb〜（2+）的特异性。获得了高度特异性的Pb〜（2+）结合肽ThrAsnThrLeuSerAsnAsn（TNTLSNN）。根据我们的知识，这是有关新型色谱生物淘洗方法与整体柱结合以选择金属离子特异性结合肽的第一份报告。可以预期，这种基于整体的色谱生物淘析技术将为高通量筛选可识别多种目标物种的亲和肽提供有前途的方法。

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