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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >On-line solid-phase extraction coupled to hydrophilic interaction chromatography-mass spectrometry for the determination of polar drugs
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On-line solid-phase extraction coupled to hydrophilic interaction chromatography-mass spectrometry for the determination of polar drugs

机译：在线固相萃取结合亲水相互作用色谱-质谱法测定极性药物

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摘要

The present study describes the first fully automated method based on on-line solid-phase extraction (SPE) coupled to hydrophilic interaction chromatography-electrospray-mass spectrometry (HILIC-(ESI)MS) to determine a group of polar drugs that includes illicit drugs (such as cocaine, morphine, codeine and metabolites) and pharmaceuticals in environmental water samples. The SPE was performed using a highly retentive polymeric sorbent. The HILIC separation was optimised and the initial high organic content of the chromatographic mobile phase, was also suitable for the proper on-line elution of the analytes retained in the SPE column and for enhancing the ESI ionisation efficiency. This method allows the loading of samples of up to 250ml of ultrapure water or 10ml of environmental water samples spiked at low ngl~(-1) levels of the analytes. The method yields near 100% recoveries for all the analytes. The method was also validated with environmental water samples with linear ranges from 5 to 1000ngl~(-1) and limits of detection ≤2ngl~(-1) for most of the compounds.
机译：本研究描述了第一个基于在线固相萃取（SPE）结合亲水相互作用色谱-电喷雾质谱（HILIC-（ESI）MS）的全自动方法，以确定一组包括非法药物的极性药物（例如可卡因，吗啡，可待因和代谢产物）和环境水样品中的药品。使用高保持性聚合物吸附剂进行SPE。 HILIC分离得到了优化，色谱流动相的初始高有机含量也适合于SPE色谱柱中保留的分析物的适当在线洗脱，并提高ESI电离效率。该方法可以加载多达250ml的超纯水或10ml的环境水样品，这些样品加标了低ngl〜（-1）的分析物。该方法对所有分析物的回收率接近100％。该方法也用线性范围为5至1000ngl〜（-1）且大多数化合物的检出限≤2ngl〜（-1）的环境水样品进行了验证。

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