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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Enhanced fluidity liquid chromatography for hydrophilic interaction separation of nucleosides
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Enhanced fluidity liquid chromatography for hydrophilic interaction separation of nucleosides

机译：增强流动性液相色谱，用于核苷的亲水相互作用分离

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摘要

The application of enhanced fluidity liquid (EFL) mobile phases to improving isocratic chromatographic separation of nucleosides in hydrophilic interaction liquid chromatography (HILIC) mode is described. The EFL mobile phase was created by adding carbon dioxide to a methanol/buffer solution. Previous work has shown that EFL mobile phases typically increase the efficiency and the speed of the separation. Herein, an increase in resolution with the addition of carbon dioxide is also observed. This increase in resolution was achieved through increased selectivity and retention with minimal change in separation efficiency. The addition of CO_2 to the mobile phase effectively decreases its polarity, thereby promoting retention in HILIC. Conventional organic solvents of similar nonpolar nature cannot be used to achieve similar results because they are not miscible with methanol and water. The separation of nucleosides with methanol/aqueous buffer/CO_2 mobile phases was also compared to that using acetonitrile/buffer mobile phases. A marked decrease in the necessary separation time was noted for methanol/aqueous buffer/CO_2 mobile phases compared to acetonitrile/buffer mobile phases. There was also an unusual reversal in the elution order of uridine and adenosine when CO_2 was included in the mobile phase.
机译：描述了增强流动性液体（EFL）流动相在亲水相互作用液相色谱（HILIC）模式下改善核苷的等度色谱分离的应用。通过将二氧化碳添加到甲醇/缓冲液中来创建EFL流动相。先前的工作表明，EFL流动相通常可以提高分离效率和分离速度。在本文中，还观察到随着添加二氧化碳分辨率的提高。分离度的提高是通过提高选择性和保留率，同时分离效率的变化最小而实现的。向流动相中添加CO_2可有效降低其极性，从而促进HILIC的保留。具有相似非极性性质的常规有机溶剂不能用于获得相似的结果，因为它们不能与甲醇和水混溶。还比较了使用甲醇/水性缓冲液/ CO_2流动相与使用乙腈/缓冲液流动相进行核苷的分离。与乙腈/缓冲液流动相相比，甲醇/缓冲水溶液/ CO_2流动相的必要分离时间明显减少。当流动相中包含CO_2时，尿苷和腺苷的洗脱顺序也发生了异常的逆转。

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