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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >On-line concentration sample stacking coupled with water-in-oil microemulsion electrokinetic chromatography
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On-line concentration sample stacking coupled with water-in-oil microemulsion electrokinetic chromatography

机译：在线浓缩样品堆叠与油包水微乳液电动色谱联用

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摘要

This study describes for the first time, the ability of a normal stacking mode (NSM) on-line concentration step coupled with water-in-oil (W/O) microemulsion electrokinetic chromatography (MEEKC), using six common penicillin antibiotics (oxacillin, penicillin V, penicillin G, nafcillin, ampicillin, and amoxicillin) as test analytes. Optimization of penicillin separation in the conventional W/O MEEKC system demonstrated that change in the type and concentration of the oil phase (1-butanol) and column temperature had a pronounced effect on the separation. With the subsequent development of the NSM coupled with W/O MEEKC, improved separation and detection sensitivities were observed when an organic solvent plug (1-propanol; 1.04. cm) was placed between the W/O microemulsion and the sample solutions. This could be attributed to the solution viscosity difference between the aqueous sample zone and the organic solvent plug causing the penicillin to be stacked in this 1-propanol plug. The optimal NSM W/O MEEKC provided about 12-fold increase in detection sensitivity compared with conventional sample injection (50. mbar, 3. s). Finally, this proposed method was successfully applied in the analyses of several food samples (porcine organs) spiked with penicillin.
机译：这项研究首次描述了使用六种常见的青霉素抗生素（奥沙西林，青霉素V，青霉素G，萘夫西林，氨苄青霉素和阿莫西林）作为测试分析物。传统W / O MEEKC系统中青霉素分离的优化表明，油相（1-丁醇）的类型和浓度以及色谱柱温度的变化对分离有显着影响。随着NSM与W / O MEEKC的后续开发，当在W / O微乳液和样品溶液之间放置有机溶剂塞（1-丙醇; 1.04。cm）时，观察到了改进的分离和检测灵敏度。这可能归因于水性样品区和有机溶剂塞之间的溶液粘度差异，导致青霉素堆积在该1-丙醇塞中。与常规样品进样（50 mbar，3 s）相比，最佳NSM W / O MEEKC可使检测灵敏度提高约12倍。最后，该方法成功地用于加标了青霉素的几种食品样品（猪器官）的分析。

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