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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Simultaneous determination of allantoin and glycolic acid in snail mucus and cosmetic creams with high performance liquid chromatography and ultraviolet detection
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Simultaneous determination of allantoin and glycolic acid in snail mucus and cosmetic creams with high performance liquid chromatography and ultraviolet detection

机译：高效液相色谱和紫外检测同时测定蜗牛粘液和美容霜中尿囊素和乙醇酸的含量

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摘要

A new methodology for simultaneous quantitative analysis of allantoin and glycolic acid in snail mucus and cosmetic creams was developed. HPLC separation was achieved a Synergi-Hydro RP column within 7 min using isocratic elution with potassium phosphate (pH 2.7; 10 mM) at a flow rate of 0.7 mL/min at 30 ℃. Sample pretreatment was performed by dilution of mucus or cosmetic cream in the elution buffer, heating at 60 ℃ for 20 min, adjusting the pH to 2.9 and purification with hexane extraction. Linearity was determined with spiked samples and the LLOQ values of 0.0125 and 0.2500 mg/mL were determined for allantoin and glycolic acid, respectively. Accuracy and intra- and inter-day repeatability were studied at three levels of concentrations (0.04, 0.08 and 0.16 mg/mL for allantoin and 0.1, 1.5 and 4.0 mg/mL for glycolic acid) using spiked mucus and cream base samples; mean values of recovery were in the range of 96.81-102.42% in all matrices tested, whereas the respective RSDs (%Relative Standard Deviation) were less than 3.04% in all cases. Spiked mucus and cream samples were stable (RSD<4.16 and relative error < 4.34%) at room temperature and at 4 ℃ for 1 week and at -18 ℃ for 6 months; samples were also stable after three freeze-thaw cycles. The method was applied to the analysis of different lots of snail mucus, and of three commercial creams containing snail mucus.
机译：开发了一种同时定量分析蜗牛黏液和美容霜中尿囊素和乙醇酸的新方法。使用磷酸钾（pH 2.7; 10 mM）等度洗脱，在30℃下以0.7 mL / min的流速在7分钟内完成Synergi-Hydro RP柱的HPLC分离。通过在洗脱缓冲液中稀释粘液或美容霜，在60℃加热20分钟，将pH值调节到2.9并用己烷萃取纯化来进行样品预处理。用加标样品测定线性，尿囊素和乙醇酸的LLOQ值分别为0.0125和0.2500 mg / mL。使用加标的粘液和乳膏基质样品在三种浓度水平（尿囊素为0.04、0.08和0.16 mg / mL，乙醇酸为0.1、1.5和4.0 mg / mL）下研究了准确性以及日内和日间重复性;在所有测试的基质中，回收率的平均值在96.81-102.42％的范围内，而在所有情况下，各自的RSD（相对标准偏差％）均小于3.04％。掺入的粘液和稀奶油样品在室温，4℃下1周和-18℃下6个月内稳定（RSD <4.16，相对误差<4.34％）;在三个冻融循环后，样品也保持稳定。该方法可用于分析不同批次的蜗牛粘液和三种含有蜗牛粘液的市售乳膏。

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