Close-packed array of gold nanoparticles and sum frequency generation spectroscopy in total internal reflection: a platform for studying biomolecules and biosensors

Tourillon G; Dreesen L; Volcke C; Sartenaer Y; Thiry P; Peremans A

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Close-packed array of gold nanoparticles and sum frequency generation spectroscopy in total internal reflection: a platform for studying biomolecules and biosensors

机译：金纳米粒子的密排阵列和全内反射的总和频率产生光谱：研究生物分子和生物传感器的平台

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摘要

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the sum frequency generation spectroscopy performed in the total internal reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers.

机译：引入了一种无需使用任何标记物即可研究生物分子的吸附和识别机制的方法。我们首次展示了在全内反射（TIR-SFG）几何形状中进行的总频率生成光谱，与规则的紧密堆积的金纳米颗粒阵列相结合，可以高灵敏度地探测构象和方向的变化由生物素对亲和素的识别过程诱导。这种方法代表了一个新平台，有望在生物传感器，诊断和生物活性层中使用。

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来源
《Journal of Materials Science》 |2009年第24期|共6页
作者
Tourillon G; Dreesen L; Volcke C; Sartenaer Y; Thiry P; Peremans A;
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正文语种 eng
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关键词
ATOMIC-FORCE MICROSCOPY; SELF-ASSEMBLED MONOLAYERS; VIBRATIONAL; SPECTROSCOPY; PROTEIN ADSORPTION; STRETCHING VIBRATIONS; SURFACE; WETTABILITY; CARBON NANOTUBES; SERUM-ALBUMIN; BIOTIN; AVIDIN;

机译：原子力显微镜;自组装单层膜;振动;光谱学;蛋白质吸附;刮擦振动;表面;可润湿性;碳纳米管;血清白蛋白;生物素;AVIDIN;

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专利

1. Close-packed array of gold nanoparticles and sum frequency generation spectroscopy in total internal reflection: a platform for studying biomolecules and biosensors [J] . Gérard Tourillon, Laurent Dreesen, Cédric Volcke, Journal of Materials Science . 2009,第24期

机译：金纳米粒子的紧密堆积阵列和全内反射的总和频率产生光谱：用于研究生物分子和生物传感器的平台
2. Total internal reflection sum-frequency generation spectroscopy and dense gold nanoparticles monolayer: a route for probing adsorbed molecules [J] . Gerard Tourillon, Laurent Dreesen, Cedric Volcke Nanotechnology . 2007,第41期

机译：全内反射和频生成光谱和致密金纳米粒子单层：探测吸附分子的途径
3. Total internal reflection sum-frequency generation spectroscopy and dense gold nanoparticles monolayer: a route for probing adsorbed molecules [J] . Tourillon G, Dreesen L, Volcke C, Nanotechnology . 2007,第41期

机译：全内反射和频生成光谱和致密金纳米粒子单层：探测吸附分子的途径
4. Conformational Order of Octadecanethiol (ODT) Monolayer at Gold/Solution Interface: Internal Reflection Sum Frequency Generation (SFG) Study [C] . Shen Ye, Satoshi Nihonyanagi, Ken Fujishima, International conference on colloid and surface science . 2001

机译：金/溶液界面的十八烷硫醇（ODT）单层的构象顺序：内反射和频生成（SFG）研究
5. In-situ studies of the reactions of bifunctional and heterocyclic molecules over noble metal single crystal and nanoparticle catalysts studied with kinetics and sum-frequency generation vibrational spectroscopy. [D] . Kliewer, Christopher Jesse. 2009

机译：用动力学和和频产生振动光谱学研究了双官能团和杂环分子在贵金属单晶和纳米颗粒催化剂上的反应的原位研究。
6. Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy. [O] . N L Thompson, T P Burghardt, D Axelrod 1981

机译：通过全内反射荧光与光漂白恢复或相关光谱法测量生物分子的表面动力学。
7. Close-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors [O] . Tourillon Gérard, Dreesen Laurent, Volcke Cédric, 2009

机译：金纳米粒子的紧密堆积阵列和全内反射的总和频率生成光谱：用于研究生物分子和生物传感器的平台

Close-packed array of gold nanoparticles and sum frequency generation spectroscopy in total internal reflection: a platform for studying biomolecules and biosensors

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