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首页> 外文期刊>Journal of nanoscience and nanotechnology >Preparation of Deep Sea Fish Oil-Based Nanostructured Lipid Carriers with Enhanced Cellular Uptake
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Preparation of Deep Sea Fish Oil-Based Nanostructured Lipid Carriers with Enhanced Cellular Uptake

机译：增强细胞摄取的深海鱼油基纳米结构脂质载体的制备

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摘要

Nanostructured lipid carriers (NLC) are a promising pharmaceutical delivery system with mean diameter less than 200 nm which are dispersed in an aqueous phase containing emulsifier(s), to increase the water solubility, stability and bioavailability of oil compounds. Herein we prepared a promising NLC with glyceryl monostearate (GMS) as the solid lipid template and deep sea fish oil as the liquid lipid template using melted-ultrasonic method. Fish oil-NLC had a mean size of 84.7 +/- 2.6 nm and a zeta potential that ranged from -17.87 mV to -32.91 mV. The nanoparticles exhibited good stability for four weeks with a high encapsulation efficiency of 87.5 +/- 5.2%. Afterwards, confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) were used to investigate the contribution of Fish oil-NLC in enhancing fluorescein isothiocyanate (FITC) cellular uptake in comparison with free FITC. The results of this study indicated the possibility of this carrier to overcome the shortcomings of deep sea fish oil and to provide a novel bifunctional carrier with nutritional potential and drug delivery ability.
机译：纳米结构脂质载体（NLC）是一种有前途的药物输送系统，平均直径小于200 nm，该系统分散在含有乳化剂的水相中，以增加油化合物的水溶性，稳定性和生物利用度。在本文中，我们采用熔融超声法制备了具有前途的NLC，其中单硬脂酸甘油酯（GMS）为固体脂质模板，深海鱼油为液体脂质模板。鱼油-NLC的平均大小为84.7 +/- 2.6 nm，ζ电位范围为-17.87 mV至-32.91 mV。纳米颗粒表现出良好的稳定性，持续四个星期，包封效率为87.5 +/- 5.2％。之后，使用共聚焦激光扫描显微镜（CLSM）和流式细胞术（FCM）来研究鱼油-NLC与游离FITC相比在增强异硫氰酸荧光素（FITC）细胞摄取方面的贡献。这项研究的结果表明该载体有可能克服深海鱼油的缺点，并提供一种具有营养潜力和药物传递能力的新型双功能载体。

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