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首页> 外文期刊>Journal of Molecular Biology >Plasticity of the gene functions for DNA replication in the T4-like phages.
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Plasticity of the gene functions for DNA replication in the T4-like phages.

机译：该基因的可塑性对T4样噬菌体中的DNA复制起作用。

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摘要

We have completely sequenced and annotated the genomes of several relatives of the bacteriophage T4, including three coliphages (RB43, RB49 and RB69), three Aeromonas salmonicida phages (44RR2.8t, 25 and 31) and one Aeromonas hydrophila phage (Aeh1). In addition, we have partially sequenced and annotated the T4-like genomes of coliphage RB16 (a close relative of RB43), A. salmonicida phage 65, Acinetobacter johnsonii phage 133 and Vibrio natriegens phage nt-1. Each of these phage genomes exhibited a unique sequence that distinguished it from its relatives, although there were examples of genomes that are very similar to each other. As a group the phages compared here diverge from one another by several criteria, including (a) host range, (b) genome size in the range between approximately 160 kb and approximately 250 kb, (c) content and genetic organization of their T4-like genes for DNA metabolism, (d) mutational drift of the predicted T4-like gene products and their regulatory sites and (e) content ofopen-reading frames that have no counterparts in T4 or other known organisms (novel ORFs). We have observed a number of DNA rearrangements of the T4 genome type, some exhibiting proximity to putative homing endonuclease genes. Also, we cite and discuss examples of sequence divergence in the predicted sites for protein-protein and protein-nucleic acid interactions of homologues of the T4 DNA replication proteins, with emphasis on the diversity in sequence, molecular form and regulation of the phage-encoded DNA polymerase, gp43. Five of the sequenced phage genomes are predicted to encode split forms of this polymerase. Our studies suggest that the modular construction and plasticity of the T4 genome type and several of its replication proteins may offer resilience to mutation, including DNA rearrangements, and facilitate the adaptation of T4-like phages to different bacterial hosts in nature.
机译：我们已经对T4噬菌体的几个亲戚的基因组进行了完整的测序和注释，包括三个噬菌体（RB43，RB49和RB69），三个嗜盐气单胞菌噬菌体（44RR2.8t，25和31）和一个嗜水气单胞菌噬菌体（Aeh1）。另外，我们已经部分测序并注释了大肠杆菌噬菌体RB16（RB43的近亲），沙门氏菌噬菌体65，约翰逊不动杆菌噬菌体133和纳氏弧菌噬菌体nt-1的T4样基因组。这些噬菌体基因组中的每一个都展示出一个独特的序列，将其与其亲属区分开来，尽管有一些非常相似的基因组实例。作为一个组，此处比较的噬菌体通过几个标准彼此不同，包括（a）宿主范围，（b）基因组大小在大约160 kb和大约250 kb之间，（c）T4的含量和遗传结构像是DNA代谢的基因，（d）预测的T4样基因产物及其调控位点的突变漂移，以及（e）T4或其他已知生物（新的ORF）中没有对应物的开放阅读框的含量。我们已经观察到T4基因组类型的许多DNA重排，其中一些表现出与推定的归巢内切核酸酶基因接近。此外，我们引用并讨论了T4 DNA复制蛋白同源物的蛋白质-蛋白质和蛋白质-核酸相互作用的预测位点中序列差异的例子，重点是噬菌体编码的序列，分子形式和调控的多样性DNA聚合酶，gp43。预计五个测序的噬菌体基因组将编码这种聚合酶的分裂形式。我们的研究表明，T4基因组类型及其几种复制蛋白的模块化构建和可塑性可能为突变提供复原力，包括DNA重排，并促进T4样噬菌体适应自然界中的不同细菌宿主。

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