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首页> 外文期刊>Journal of Molecular Biology >A cytoskeletal demolition worker: Myosin II acts as an actin depolymerization agent
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A cytoskeletal demolition worker: Myosin II acts as an actin depolymerization agent

机译：细胞骨架拆卸工人：肌球蛋白II充当肌动蛋白解聚剂

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Myosin II motors play several important roles in a variety of cellular processes, some of which involve active assembly/disassembly of cytoskeletal substructures. Myosin II motors have been shown to function in actin bundle turnover in neuronal growth cones and in the recycling of actin filaments during cytokinesis. Close examination had shown an intimate relationship between myosin II motor adenosine triphosphatase activity and actin turnover rate. However, the direct implication of myosin II in actin turnover is still not understood. Herein, we show, using high-resolution cryo-transmission electron microscopy, that myosin II motors control the turnover of actin bundles in a concentration-dependent manner in vitro. We demonstrate that disassembly of actin bundles occurs through two main stages: the first stage involves unbundling into individual filaments, and the second involves their subsequent depolymerization. These evidence suggest that, in addition to their "classical" contractile abilities, myosin II motors may be directly implicated in active actin depolymerization. We believe that myosin II motors may function similarly in vivo (e.g., in the disassembly of the contractile ring by fine tuning the local concentration/ activity of myosin II motors). (C) 2007 Elsevier Ltd. All rights reserved.
机译：肌球蛋白II马达在多种细胞过程中起着重要作用，其中一些涉及细胞骨架亚结构的主动组装/拆卸。肌球蛋白II马达已显示出在神经元生长锥中的肌动蛋白束更新和胞质分裂过程中肌动蛋白丝回收的功能。仔细检查显示，肌球蛋白II运动型腺苷三磷酸酶活性与肌动蛋白周转率之间存在密切关系。然而，肌球蛋白II在肌动蛋白周转率中的直接含义仍不清楚。在这里，我们显示，使用高分辨率的低温透射电子显微镜，肌球蛋白II电机在体外以浓度依赖的方式控制肌动蛋白束的周转。我们证明肌动蛋白束的拆卸经历了两个主要阶段：第一个阶段涉及解开成单个的细丝，第二个阶段涉及其随后的解聚。这些证据表明，除了它们的“经典”收缩能力外，肌球蛋白II马达还可能与活性肌动蛋白解聚直接相关。我们认为，肌球蛋白II马达可能在体内具有类似的功能（例如，通过微调肌球蛋白II马达的局部浓度/活性来分解收缩环）。 （C）2007 Elsevier Ltd.保留所有权利。

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